Seed Dormancy

Methods and Protocols

  • Allison R. Kermode

Part of the Methods in Molecular Biology book series (MIMB, volume 773)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. M. Verónica Rodríguez, Peter E. Toorop, Roberto L. Benech-Arnold
      Pages 17-40
  3. Breaking Seed Dormancy and Standardizing Seed Dormancy Research

    1. Front Matter
      Pages 41-41
    2. Henk W. M. Hilhorst
      Pages 43-52
  4. Hormone Analyses

    1. Front Matter
      Pages 81-81
    2. Xiao-Fang Wang, Qi Xin, Yuan-Yue Shen, Da-Peng Zhang
      Pages 83-97
    3. Mitsunori Seo, Yusuke Jikumaru, Yuji Kamiya
      Pages 99-111
    4. Akira Endo, Mitsuhiro Kimura, Naoto Kawakami, Eiji Nambara
      Pages 135-147
  5. Gene, RNA, and Protein Analyses

    1. Front Matter
      Pages 149-149
    2. Urszula Piskurewicz, Luis Lopez-Molina
      Pages 151-164
    3. Tiehan Zhao, Ying Zeng, Allison R. Kermode
      Pages 185-198
    4. M. B. Arun Kumar, Ruth C. Martin, Hiro Nonogaki
      Pages 199-213
    5. Loïc Rajjou, Maya Belghazi, Julie Catusse, Laurent Ogé, Erwann Arc, Béatrice Godin et al.
      Pages 215-236
  6. Epigenetic Changes Associated with Seed Dormancy

    1. Front Matter
      Pages 237-237
    2. Yongxiu Liu, Regina Geyer, Vittoria Brambilla, Kazumi Nakabayashi, Wim J. J. Soppe
      Pages 239-257
  7. Spectroscopic and Whole Tissue Analyses

    1. Front Matter
      Pages 305-305
    2. Victor Terskikh, Allison R. Kermode
      Pages 307-318
    3. Victor Terskikh, Kerstin Müller, Allison R. Kermode, Gerhard Leubner-Metzger
      Pages 319-327
    4. Wioletta E. Pluskota, Kent J. Bradford, Hiro Nonogaki
      Pages 329-339
  8. Analyses of Reactive Oxygen Species, Protein Damage and Repair

    1. Front Matter
      Pages 341-341
    2. Laurent Ogé, Caroline Broyart, Boris Collet, Béatrice Godin, Denis Jallet, Gildas Bourdais et al.
      Pages 369-384
    3. Paul C. Bethke, Igor G. L. Libourel, Jan Vitecek, Russell L. Jones
      Pages 385-400
  9. Bioinformatic Resources

    1. Front Matter
      Pages 401-401
    2. George W. Bassel, Michael J. Holdsworth, Nicholas J. Provart
      Pages 403-419
  10. Back Matter
    Pages 421-423

About this book


Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world’s food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8’-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Seed Dormancy: Methods and Protocols features detailed methods that will prove invaluable for both applied and fundamental seed research.


ABAR Abscisic acid Arabidopsis Nuclear Magnetic Resonance dormancy cycles microRNAs seed bioinformatics

Editors and affiliations

  • Allison R. Kermode
    • 1
  1. 1.Dept. Biological SciencesSimon Fraser UnviersityBurnabyCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-230-4
  • Online ISBN 978-1-61779-231-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site