In Vitro Neurotoxicology

Methods and Protocols

  • Lucio G. Costa
  • Gennaro Giordano
  • Marina Guizzetti

Part of the Methods in Molecular Biology book series (MIMB, volume 758)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Model Systems

    1. Front Matter
      Pages 11-11
    2. Lucio G. Costa, Gennaro Giordano, Marina Guizzetti
      Pages 1-9
  3. Model Systems

    1. Front Matter
      Pages 11-11
    2. Evelyn Tiffany-Castiglioni, Yongchang Qian, Rola Barhoumi, Ying Zheng
      Pages 29-48
    3. Anke Witting, Thomas Möller
      Pages 49-66
    4. Roshan Tofighi, Michaela Moors, Raj Bose, Wan Norhamidah Wan Ibrahim, Sandra Ceccatelli
      Pages 67-80
    5. Paul Honegger, Antoinette Defaux, Florianne Monnet-Tschudi, Marie-Gabrielle Zurich
      Pages 81-97
    6. Ellen Fritsche, Kathrin Gassmann, Timm Schreiber
      Pages 99-114
    7. Pamela J. Lein, Christopher D. Barnhart, Isaac N. Pessah
      Pages 115-134
    8. Hilary Vernon, Katherine Clark, Joseph P. Bressler
      Pages 153-168
  4. Cell Death

    1. Front Matter
      Pages 169-169
    2. Gennaro Giordano, Sungwoo Hong, Elaine M. Faustman, Lucio G. Costa
      Pages 171-178
    3. Gennaro Giordano, Lucio G. Costa
      Pages 179-193
    4. Dejan Milatovic, Thomas J. Montine, Michael Aschner
      Pages 195-204
    5. Gennaro Giordano, Collin C. White, Lucio G. Costa
      Pages 205-214
    6. Stephanie L. H. Zeiger, Jeannette N. Stankowski, BethAnn McLaughlin
      Pages 215-235
  5. Signaling in Neurotoxicity

    1. Front Matter
      Pages 237-237
    2. David P. Cox, Collin C. White, Terrance J. Kavanagh
      Pages 239-251
    3. Ronald B. Tjalkens, David L. Carbone, Guoyao Wu
      Pages 267-277
    4. Won-Seok Choi, Heather M. Klintworth, Zhengui Xia
      Pages 279-292
    5. Faneng Sun, Anumantha G. Kanthasamy, Arthi Kanthasamy
      Pages 293-305
  6. Other End-Points

    1. Front Matter
      Pages 329-329
    2. Joshua A. Harrill, William R. Mundy
      Pages 331-348
    3. Marina Guizzetti, Terrance J. Kavanagh, Lucio G. Costa
      Pages 349-359
    4. Pamela J. Roqué, Marina Guizzetti, Gennaro Giordano, Lucio G. Costa
      Pages 361-390
    5. Jing Chen, Lucio G. Costa, Marina Guizzetti
      Pages 403-414
  7. Back Matter
    Pages 435-444

About this book


In recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems.  In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems.  Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals.


Cell signaling impairment Cellular death Chemicals Drugs Environmental contaminants Mammalian cell culture Model systems Nervous system cell toxicity Neurotoxicity Pharmaceuticals

Editors and affiliations

  • Lucio G. Costa
    • 1
  • Gennaro Giordano
    • 2
  • Marina Guizzetti
    • 3
  1. 1., Department of Environmental andUniversity of WashingtonSeattleUSA
  2. 2., Department of Environmental andUniversity of WashingtonSeattleUSA
  3. 3.Jesse Brown VA Medical Center, Department of PsychiatryUniversity of Illinois at ChicagoSeattleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-169-7
  • Online ISBN 978-1-61779-170-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site