Plant Transcription Factors

Methods and Protocols

  • Ling Yuan
  • Sharyn E. Perry

Part of the Methods in Molecular Biology book series (MIMB, volume 754)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Rainer Melzer, Günter Theißen
      Pages 3-18
  3. Identification of Transcription Factors

  4. Functional Verification

    1. Front Matter
      Pages 85-85
    2. Nobutaka Mitsuda, Kyoko Matsui, Miho Ikeda, Masaru Nakata, Yoshimi Oshima, Yukari Nagatoshi et al.
      Pages 87-105
    3. Yuji Iwata, Mi-Hyun Lee, Nozomu Koizumi
      Pages 107-117
    4. Maartje Gorte, Anneke Horstman, Robert B. Page, Renze Heidstra, Arnold Stromberg, Kim Boutilier
      Pages 119-141
  5. Protein–Protein Interaction and Other Means for Activity Control

    1. Front Matter
      Pages 143-143
    2. Stefan de Folter, Richard G.H. Immink
      Pages 145-165
    3. Ling Zhu, Enamul Huq
      Pages 167-184
    4. Jelle Van Leene, Dominique Eeckhout, Geert Persiau, Eveline Van De Slijke, Jan Geerinck, Gert Van Isterdael et al.
      Pages 195-218
    5. Jasmina Kurepa, Jan A. Smalle
      Pages 219-234
    6. Munawar Ahmad, Won Kyong Cho, Yeonggil Rim, Lijun Huang, Jae-Yean Kim
      Pages 235-245
  6. Protein–DNA Interaction

  7. Engineering Metabolic Pathways Using Transcription Factors

    1. Front Matter
      Pages 323-323
  8. Back Matter
    Pages 343-347

About this book


Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors. 


DNA shuffling arabidopsis protoplasts chimeric repressors direct target genes gene discovery protein-DNA interactions protein-protein interactions yeast one-hybrid systems

Editors and affiliations

  • Ling Yuan
    • 1
  • Sharyn E. Perry
    • 2
  1. 1.College of Agriculture, Dept. Plant & Soil SciencesUniversity of KentuckyLexingtonUSA
  2. 2.Dept. Plant & Soil SciencesUniversity of KentuckyLexingtonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-153-6
  • Online ISBN 978-1-61779-154-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site