Bioconjugation Protocols

Strategies and Methods

  • Sonny S. Mark

Part of the Methods in Molecular Biology book series (MIMB, volume 751)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Protein Conjugates

    1. Front Matter
      Pages 1-1
    2. Ahmed Besheer, Thomas C. Hertel, Jörg Kressler, Karsten Mäder, Markus Pietzsch
      Pages 17-27
    3. Sukanta Dolai, Wei Shi, Bikash Mondal, Krishnaswami Raja
      Pages 29-42
    4. Mathieu Galibert, Olivier Renaudet, Didier Boturyn, Pascal Dumy
      Pages 67-79
    5. Anna Mero, Chiara Clementi, Francesco M. Veronese, Gianfranco Pasut
      Pages 95-129
  3. Nucleic Acid Conjugates

    1. Front Matter
      Pages 143-143
    2. Andrea Masotti, Francesco Pampaloni
      Pages 145-165
    3. Gwladys Pourceau, Albert Meyer, Jean-Jacques Vasseur, François Morvan
      Pages 167-193
    4. Samuel H. Weisbrod, Anna Baccaro, Andreas Marx
      Pages 195-207
    5. Anna Aviñó, Santiago Grijalvo, Sónia Pérez-Rentero, Alejandra Garibotti, Montserrat Terrazas, Ramon Eritja
      Pages 223-238
    6. Jan Zimmermann, Minseok Kwak, Andrew J. Musser, Andreas Herrmann
      Pages 239-266
  4. Glycosyl and Lipid Conjugates

    1. Front Matter
      Pages 267-267
    2. Hailong Zhang, Yong Ma, Xue-Long Sun
      Pages 269-280

About this book


Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology.  In this second edition of Bioconjugation Protocols:  Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering.  These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches.  Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices).  All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.


Biofunctionalization Biomaterials engineering Biomedical diagnostics Labeling techniques Nanomaterials Organic synthesis Surface biotechnology Therapeutics

Editors and affiliations

  • Sonny S. Mark
    • 1
  1. 1.Pacific BiosciencesMenlo ParkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-150-5
  • Online ISBN 978-1-61779-151-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book