Stem Cell Migration

Methods and Protocols

  • Marie-Dominique Filippi
  • Hartmut Geiger

Part of the Methods in Molecular Biology book series (MIMB, volume 750)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Stem Cell and Migration: An Overview

    1. Front Matter
      Pages 1-1
    2. Claire Magnon, Daniel Lucas, Paul S. Frenette
      Pages 3-24
    3. David A. Hess, Alison L. Allan
      Pages 25-44
  3. Stem Cell Identification and Microscopic Technologies to Track Transplanted Stem Cells In Vivo

    1. Front Matter
      Pages 45-45
    2. Lara Rossi, Grant A. Challen, Olga Sirin, Karen Kuan-Yin Lin, Margaret A. Goodell
      Pages 47-59
    3. Florian A. Siebzehnrubl, Vinata Vedam-Mai, Hassan Azari, Brent A. Reynolds, Loic P. Deleyrolle
      Pages 61-77
    4. Eva Syková, Pavla Jendelová, Vít Herynek
      Pages 79-90
    5. Rahul Kasukurthi, Terence M. Myckatyn
      Pages 91-100
    6. Andrew S. Lee, Joseph C. Wu
      Pages 101-114
  4. Stem Cell Migration During Development

    1. Front Matter
      Pages 115-115
    2. Brian Dudley, Kathleen Molyneaux
      Pages 117-129
    3. Jin-Wu Tsai, Richard B. Vallee
      Pages 131-142
    4. Michael J. Ferkowicz, Mervin C. Yoder
      Pages 143-155
    5. Pulin Li, Leonard I. Zon
      Pages 157-168
    6. Michael J. Murray, Robert Saint
      Pages 169-184
  5. Adult Hematopoietic Stem Cell Migration

About this book

Introduction

The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function.  Due to the low frequency of these cells in vivo,  problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently.  In Stem Cell Migration: Methods and Protocols, experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and convenient, Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.

Keywords

Cancer stem cells Embryogenesis In vivo technologies Migration and disease Organ regeneration Signaling pathways Tissue homeostasis

Editors and affiliations

  • Marie-Dominique Filippi
    • 1
  • Hartmut Geiger
    • 2
  1. 1.Medical Center, Division of Experimental HematologyCincinnati Children's HospitalCincinnatiUSA
  2. 2.Medical Center, Division of Experimental HematologyCincinnati Children's HospitalCincinnatiUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-145-1
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-144-4
  • Online ISBN 978-1-61779-145-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book