DNA Recombination

Methods and Protocols

  • Hideo Tsubouchi

Part of the Methods in Molecular Biology book series (MIMB, volume 745)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Genetic and Molecular Biological Approaches with Yeast

    1. Front Matter
      Pages 1-1
    2. Xiuzhong Zheng, Anastasiya Epstein, Hannah L. Klein
      Pages 3-13
    3. Wenjian Ma, Jim Westmoreland, Wataru Nakai, Anna Malkova, Michael A. Resnick
      Pages 15-31
    4. Scott Houghtaling, Toyoko Tsukuda, Mary Ann Osley
      Pages 79-97
    5. Stacy Y. Chen, Jennifer C. Fung
      Pages 117-134
    6. Belén Gómez-González, José F. Ruiz, Andrés Aguilera
      Pages 151-172
    7. Ying Shen, Francesca Storici
      Pages 193-204
  3. Genetic and Molecular Biological Approaches with Higher Eukaryotes

    1. Front Matter
      Pages 205-205
    2. Grace C. Bazan, Kenneth J. Hillers
      Pages 207-222
    3. Francesca Cole, Maria Jasin
      Pages 251-282
    4. Koji Nakanishi, Francesca Cavallo, Erika Brunet, Maria Jasin
      Pages 283-291
    5. Hiroyuki Kitao, Seiki Hirano, Minoru Takata
      Pages 293-309
    6. Vera Batrak, Artem Blagodatski, Jean-Marie Buerstedde
      Pages 311-326
  4. In Vitro Reconstitution of Homologous Recombination Reactions and Single Molecular Analysis of Recombination Proteins

    1. Front Matter
      Pages 327-327
    2. Xiao-Ping Zhang, Wolf-Dietrich Heyer
      Pages 329-343
    3. William D. Wright, Kirk T. Ehmsen, Wolf-Dietrich Heyer
      Pages 345-362
    4. Jie Liu, Jessica Sneeden, Wolf-Dietrich Heyer
      Pages 363-383
    5. Matthew J. Rossi, Dmitry V. Bugreev, Olga M. Mazina, Alexander V. Mazin
      Pages 407-420
    6. Youngho Kwon, Weixing Zhao, Patrick Sung
      Pages 421-435
    7. Yoshitami Hashimoto, Vincenzo Costanzo
      Pages 437-445
    8. Michael G. Sehorn, Hilarie A. Sehorn
      Pages 485-496
  5. Cell Biological Approaches to Study the In Vivo Behavior of Homologous Recombination

    1. Front Matter
      Pages 497-497
    2. Imen Lassadi, Kerstin Bystricky
      Pages 499-522
    3. Nadine Eckert-Boulet, Rodney Rothstein, Michael Lisby
      Pages 523-536
    4. Harry Scherthan, Caroline Adelfalk
      Pages 537-548
  6. Back Matter
    Pages 563-565

About this book


Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination. Divided into four convenient sections, DNA Recombination: Methods and Protocols covers recent techniques that best utilize the advantages of the yeast system, prescribing to the belief that yeast will keep serving as a great model organism to study homologous recombination. Chapters have also been included for such exceptions as the group of genes involved in recombination that are found solely in higher eukaryotes, such as BRCA2. And looking forward, a necessary step in the direction of understanding the homologous recombination process is to isolate the machine and let it work in a test tube.  Understanding the design by studying the appearance and behavior of the machinery as a single molecule will be an important milestone toward understanding the mechanism of action of the machinery. Techniques covering these topics have also been included.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, DNA Recombination: Methods and Protocols serves as an ideal guide to scientists of all backgrounds with its well-honed methodologies and strives to bring the reader to the next level of understanding regarding this vital subject.


DNA double-strand break DNA microarrays genome stability higher eukaryotes homologous recombination meiosis yeast genetics

Editors and affiliations

  • Hideo Tsubouchi
    • 1
  1. 1.MRC Genome Damage and Stability CentreUniversity of SussexBrightonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-128-4
  • Online ISBN 978-1-61779-129-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site