Cystic Fibrosis

Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis

  • Margarida D. Amaral
  • Karl Kunzelmann

Part of the Methods in Molecular Biology book series (MIMB, volume 742)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Pathophysiology of Cystic Fibrosis

    1. Front Matter
      Pages 1-1
    2. Silvia M. Kreda, Martina Gentzsch
      Pages 15-33
    3. Yawar J. Qadri, Estelle Cormet-Boyaka, Dale J. Benos, Bakhrom K. Berdiev
      Pages 35-50
    4. Assem G. Ziady, Pamela B. Davis
      Pages 51-76
    5. Erin N. Worthington, Robert Tarran
      Pages 77-92
    6. Jeffrey J. Wine, Nam Soo Joo, Jae Young Choi, Hyung-Ju Cho, Mauri E. Krouse, Jin V. Wu et al.
      Pages 93-112
    7. Kristina A. Thomsson, Gunnar C. Hansson
      Pages 127-141
    8. Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena Nørgaard, Niels Høiby
      Pages 143-171
    9. Sophie Crespin, Marc Bacchetta, Song Huang, Tecla Dudez, Ludovic Wiszniewski, Marc Chanson
      Pages 173-185
  3. Omic Approaches to Study Cystic Fibrosis

    1. Front Matter
      Pages 187-187
    2. Shyam Ramachandran, Luka A. Clarke, Todd E. Scheetz, Margarida D. Amaral, Paul B. McCray Jr
      Pages 193-212
    3. Ida Chiara Guerrera, Mario Ollero, Diane-Lore Vieu, Aleksander Edelman
      Pages 213-225
    4. Joana Almaça, Shehrazade Dahimène, Nicole Appel, Christian Conrad, Karl Kunzelmann, Rainer Pepperkok et al.
      Pages 249-264
    5. Mario Ollero, Ida Chiara Guerrera, Giuseppe Astarita, Daniele Piomelli, Aleksander Edelman
      Pages 265-278
  4. Resources

    1. Front Matter
      Pages 279-279
    2. Scott H. Randell, M. Leslie Fulcher, Wanda O’Neal, John C. Olsen
      Pages 285-310
    3. John T. Fisher, Yulong Zhang, John F. Engelhardt
      Pages 311-334
    4. Kathryn W. Peters, Tsukasa Okiyoneda, William E. Balch, Ineke Braakman, Jeffrey L. Brodsky, William B. Guggino et al.
      Pages 335-353
    5. Patrick R. Sosnay, Carlo Castellani, Mary Corey, Ruslan Dorfman, Julian Zielenski, Rachel Karchin et al.
      Pages 355-372
  5. Back Matter
    Pages 373-384

About this book


Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.  Cystic Fibrosis: Diagnosis and Protocols aims to provide the CF research community and related researchers with a very wide range of high-quality experimental tools, as an easy way to grasp and use classical and novel methods applied to cystic fibrosis.  Volume II: Methods and Resources to Understand Cystic Fibrosis focuses on pathophysiology, Omics approaches, and a variety of key resources recently made available for CF research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and practical, Cystic Fibrosis: Diagnosis and Protocols will provide readers with optimal working tools to address pressing questions in the best technical way, while helping all of us, as a research and clinical community, to move faster hand-in-hand toward unravelling the secrets of this challenging disorder and cure it.


CF Genomics Human clinical trials Lipidomics Pathophysiology Proteomics

Editors and affiliations

  • Margarida D. Amaral
    • 1
  • Karl Kunzelmann
    • 2
  1. 1.Faculty of Sciences, Centre for Biodiversity & FunctionalUniversity of LisboaLisboaPortugal
  2. 2., Department of PhysiologyUniversity of RegensburgRegensburgGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-119-2
  • Online ISBN 978-1-61779-120-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site