Mammalian Cell Viability

Methods and Protocols

  • Martin J. Stoddart

Part of the Methods in Molecular Biology book series (MIMB, volume 740)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Martin J. Stoddart
    Pages 1-6
  3. Kristine S. Louis, Andre C. Siegel
    Pages 7-12
  4. Ning Ke, Xiaobo Wang, Xiao Xu, Yama A. Abassi
    Pages 33-43
  5. Maya Zimmermann, Norbert Meyer
    Pages 57-63
  6. Gabriela Paroni, Claudio Brancolini
    Pages 65-79
  7. Donald Wlodkowic, Joanna Skommer, Zbigniew Darzynkiewicz
    Pages 81-89
  8. Helga H. J. Gerets, Stéphane Dhalluin, Franck A. Atienzar
    Pages 91-101
  9. Terry L. Riss, Richard A. Moravec, Andrew L. Niles
    Pages 103-114
  10. Heather M. Powell, Alexis D. Armour, Steven T. Boyce
    Pages 115-126
  11. Benjamin Gantenbein-Ritter, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, Sibylle Grad
    Pages 127-140
  12. Christian J. Stork, Yang V. Li
    Pages 157-163
  13. Karim Oudina, Adeline Cambon-Binder, Delphine Logeart-Avramoglou
    Pages 165-178
  14. Sophie Verrier, Alina Zoladek, Ioan Notingher
    Pages 179-189

About this book

Introduction

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

 

Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Keywords

3 dimensional culture systems Apoptotic or necrotic cell death Automated image analysis Cell survival Live and dead cells Monolayer and suspension cells Multiplexing assays Tissue sections

Editors and affiliations

  • Martin J. Stoddart
    • 1
  1. 1.AO Research InstituteDavos PlatzSwitzerland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-108-6
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-107-9
  • Online ISBN 978-1-61779-108-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book