Viral Vectors for Gene Therapy

Methods and Protocols

  • Otto-Wilhelm Merten
  • Mohamed Al-Rubeai

Part of the Methods in Molecular Biology book series (MIMB, volume 737)

Table of contents

  1. Front Matter
    Pages i-xi
  2. James N. Warnock, Claire Daigre, Mohamed Al-Rubeai
    Pages 1-25
  3. Patrice P. Denèfle
    Pages 27-44
  4. Glyn N. Stacey, Otto-Wilhelm Merten
    Pages 45-88
  5. María Mercedes Segura, Amine A. Kamen, Alain Garnier
    Pages 89-116
  6. Miguel Chillon, Ramon Alemany
    Pages 117-138
  7. Pedro E. Cruz, Teresa Rodrigues, Marlene Carmo, Dagmar Wirth, Ana I. Amaral, Paula M. Alves et al.
    Pages 157-182
  8. Marc Giry-Laterrière, Els Verhoeyen, Patrick Salmon
    Pages 183-209
  9. Mauro Mezzina, Otto-Wilhelm Merten
    Pages 211-234
  10. Kari J. Airenne, Kaisa-Emilia Makkonen, Anssi J. Mähönen, Seppo Ylä-Herttuala
    Pages 279-301
  11. Cornel Fraefel, Peggy Marconi, Alberto L. Epstein
    Pages 303-343
  12. Kirsten K. Langfield, Henry J. Walker, Linda C. Gregory, Mark J. Federspiel
    Pages 345-366
  13. Cecilia Frecha, Floriane Fusil, François-Loïc Cosset, Els Verhoeyen
    Pages 367-390
  14. Michael Fuchs
    Pages 407-424
  15. Back Matter
    Pages 443-450

About this book


The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular Biology™ series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.


Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.


Adeno-associated viruses Adenoviruses Baculoviruses Gene function Gene products Herpes simplex viruses Poxviruses Retroviruses Therapeutic genes

Editors and affiliations

  • Otto-Wilhelm Merten
    • 1
  • Mohamed Al-Rubeai
    • 2
  1. 1.GénéthonÉvry CX 2France
  2. 2.Chemical & Bioprocess EngineeringUniversity College DublinBelfield, DublinIreland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-094-2
  • Online ISBN 978-1-61779-095-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site