Cancer Cell Culture

Methods and Protocols

  • Ian A. Cree

Part of the Methods in Molecular Biology book series (MIMB, volume 731)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Ian A. Cree
    Pages 1-11
  3. Ian A. Cree
    Pages 13-26
  4. Katharine A. Parker
    Pages 27-34
  5. Wilhelm G. Dirks, Hans G. Drexler
    Pages 45-55
  6. Roderick A. F. MacLeod, Maren Kaufmann, Hans G. Drexler
    Pages 57-78
  7. Glyn N. Stacey
    Pages 79-91
  8. Cord C. Uphoff, Hans G. Drexler
    Pages 105-114
  9. Louise A. Knight, Ian A. Cree
    Pages 115-124
  10. Laura Breen, Joanne Keenan, Martin Clynes
    Pages 125-133
  11. Sharon Glaysher, Ian A. Cree
    Pages 135-140
  12. Julia K. Soo, Alastair D. MacKenzie Ross, Dorothy C. Bennett
    Pages 141-150
  13. Karin J. Purdie, Celine Pourreyron, Andrew P. South
    Pages 151-159
  14. Christian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, Jutta A. Kurbacher, Ralf Reichelt et al.
    Pages 161-180
  15. Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau, Roland S. Croner, Michael Stürzl
    Pages 209-218
  16. Venil N. Sumantran
    Pages 219-236

About this book

Introduction

With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.

Keywords

Cancer Cell Biology Cancer Cell Isolation Cancer Cell Lines Cancer Therapy Cell Modification Functional Assays Oncology

Editors and affiliations

  • Ian A. Cree
    • 1
  1. 1.Translation Oncology Research CentreQueen Alexandra HospitalPortsmouthUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-080-5
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-079-9
  • Online ISBN 978-1-61779-080-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book