cDNA Libraries

Methods and Applications

  • Chaofu Lu
  • John Browse
  • James G. Wallis

Part of the Methods in Molecular Biology book series (MIMB, volume 729)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Enhanced Approaches to Inherent Problems

    1. Front Matter
      Pages 1-1
    2. Richard H. Maier, Christina J. Maier, Kamil Önder
      Pages 71-84
    3. Ekaterina A. Bogdanova, Ekaterina V. Barsova, Irina A. Shagina, Alexander Scheglov, Veronika Anisimova, Laura L. Vagner et al.
      Pages 85-98
  3. Visionary Applications

  4. Back Matter
    Pages 271-275

About this book


The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.  In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries.  The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology.


Bioinformatic approaches Clones Complex tissue analysis In vitro recombination methods Small RNA Transcriptome

Editors and affiliations

  • Chaofu Lu
    • 1
  • John Browse
    • 2
  • James G. Wallis
    • 3
  1. 1., Dept. Plant Sciences & Plant PathologyMontana State UniversityBozemanUSA
  2. 2.Institute of Biological ChemistryWashington State UniversityPullmanUSA
  3. 3.Institute of Biological ChemistryWashington State UniversityPullmanUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-064-5
  • Online ISBN 978-1-61779-065-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site