Formalin-Fixed Paraffin-Embedded Tissues

Methods and Protocols

  • Fahd Al-Mulla

Part of the Methods in Molecular Biology book series (MIMB, volume 724)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Catherine M. With, David L. Evers, Jeffrey T. Mason
    Pages 1-21
  3. Carol B. Fowler, Yan-Gao Man, Shimin Zhang, Timothy J. O’Leary, Jeffrey T. Mason, Robert E. Cunningham
    Pages 23-35
  4. Anthony S.-Y. Leong, Trishe Y.-M. Leong
    Pages 37-68
  5. Anthony S.-Y. Leong, Zenobia Haffajee
    Pages 79-89
  6. Christian Schunck, Eiman Mohammad
    Pages 91-103
  7. Marianne Tuefferd, An de Bondt, Ilse Van den Wyngaert, Willem Talloen, Hinrich Göhlmann
    Pages 147-160
  8. Mira Bosso, Fahd Al-Mulla
    Pages 161-180
  9. Brendan Doyle, Ciarán O’Riain, Kim Appleton
    Pages 181-190
  10. Ashraf Dallol, Waleed Al-Ali, Amina Al-Shaibani, Fahd Al-Mulla
    Pages 191-204
  11. Mei-Lan Liu, Jennie Jeong, Ranjana Ambannavar, Carl Millward, Frederick Baehner, Chithra Sangli et al.
    Pages 205-237
  12. Aaron Scott, Ranjana Ambannavar, Jennie Jeong, Mei-Lan Liu, Maureen T. Cronin
    Pages 239-257
  13. Carol B. Fowler, Timothy J. O’Leary, Jeffrey T. Mason
    Pages 281-295
  14. Amélie Denouël, Florence Boissière-Michot, Philippe Rochaix, Frédéric Bibeau, Nathalie Boulle
    Pages 297-307

About this book


Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.


Cellular Biology Proteomics Tailored Disease Therapy Tissue Fixation Tissue Microarrays

Editors and affiliations

  • Fahd Al-Mulla
    • 1
  1. 1.Health Science Center, Dept. of PathologyKuwait UniversitySafatKuwait

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-054-6
  • Online ISBN 978-1-61779-055-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book