Antiviral RNAi

Concepts, Methods, and Applications

  • Ronald P. van Rij

Part of the Methods in Molecular Biology book series (MIMB, volume 721)

Table of contents

  1. Front Matter
    Pages i-xi
  2. General Introduction

    1. Front Matter
      Pages 1-1
    2. Joël T. van Mierlo, Koen W. R. van Cleef, Ronald P. van Rij
      Pages 3-22
    3. Joost Haasnoot, Ben Berkhout
      Pages 23-41
    4. Karlie Plaisance-Bonstaff, Rolf Renne
      Pages 43-66
    5. Jiehua Zhou, John J. Rossi
      Pages 67-75
    6. Jesper B. Bramsen, Jørgen Kjems
      Pages 77-103
  3. Viral Small RNAs

    1. Front Matter
      Pages 105-105
    2. Valérie Gausson, Maria-Carla Saleh
      Pages 107-122
    3. Adam Grundhoff
      Pages 143-152
    4. Lydia V. McClure, Yao-Tang Lin, Christopher S. Sullivan
      Pages 153-171
    5. Matthias John, Sébastien Pfeffer
      Pages 173-182
    6. Ning Xu, Göran Akusjärvi
      Pages 183-198
  4. RNAi-Based Antiviral Defense

    1. Front Matter
      Pages 199-199
    2. Koen W. R. van Cleef, Joël T. van Mierlo, Marius van den Beek, Ronald P. van Rij
      Pages 201-213
    3. Baojun Yang, Hongwei Li
      Pages 215-229
    4. Arabinda Nayak, Raul Andino
      Pages 231-244
    5. Tibor Csorba, József Burgyán
      Pages 245-252
    6. Benjamin Obadia, Maria-Carla Saleh
      Pages 253-263
  5. Antiviral RNAi Therapy

    1. Front Matter
      Pages 265-265
    2. Diana Rothe, Erik J. Wade, Jens Kurreck
      Pages 267-292
    3. Ying Poi Liu, Jan-Tinus Westerink, Olivier ter Brake, Ben Berkhout
      Pages 293-311
    4. Scot D. Henry, Quiwei Pan, Luc J. W. van der Laan
      Pages 313-332
    5. Sailen Barik
      Pages 333-338
    6. Sang-Soo Kim, Sandesh Subramanya, Dan Peer, Motomu Shimaoka, Premlata Shankar
      Pages 339-353
    7. Jiehua Zhou, John J. Rossi
      Pages 355-371
  6. RNAi Screens to Study Virus–Host Interactions

  7. Scot D. Henry, Quiwei Pan, Luc J. W. van der Laan
    Pages E1-E1
  8. Back Matter
    Pages 407-411

About this book


Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.


RNAi-based antiviral drugs antiviral therapeutics siRNA delivery and stability target genes viruses

Editors and affiliations

  • Ronald P. van Rij
    • 1
  1. 1.Inst. for Infection, Inflammation & Immu, Dept. Medical MicrobiologyRadboud Univ.Nijmegen Medical CtrNijmegenNetherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-036-2
  • Online ISBN 978-1-61779-037-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site