Signal Transduction Immunohistochemistry

Methods and Protocols

  • Alexander E. Kalyuzhny

Part of the Methods in Molecular Biology book series (MIMB, volume 717)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Antibodies as a Tool: From Concept to Design and Application

    1. Front Matter
      Pages 1-1
    2. Kathy Brumbaugh, Wade Johnson, Wen-Chieh Liao, Mong-Shang Lin, J. P. Houchins, Jeff Cooper et al.
      Pages 3-43
    3. Biji T. Kurien, Yaser Dorri, Skyler Dillon, Anil Dsouza, R. Hal Scofield
      Pages 55-67
    4. Amy J. Archuleta, Crystal A. Stutzke, Kristin M. Nixon, Michael D. Browning
      Pages 69-88
  3. Preservation and Unmasking of Tissue Antigens

  4. Imaging Techniques and High-Throughput Data Analysis

    1. Front Matter
      Pages 111-111
    2. Jerry Sedgewick
      Pages 113-142
    3. Maria A. Suni, Vernon C. Maino
      Pages 155-169
    4. Jayant Avva, Michael C. Weis, Radina P. Soebiyanto, James W. Jacobberger, Sree N. Sreenath
      Pages 171-193
  5. Neuroscience, Cancer, and Stem Cell Research

    1. Front Matter
      Pages 195-195
    2. Jillian Frisch, J. P. Houchins, Michael Grahek, Jordan Schoephoerster, Jodi Hagen, Joseph Sweet et al.
      Pages 233-244
    3. Pravin J. Mishra, Debabrata Banerjee
      Pages 245-253
  6. Novel Assays and Techniques

    1. Front Matter
      Pages 255-255
    2. Christianne Bandeira-Melo, Peter F. Weller, Patricia T. Bozza
      Pages 277-289
    3. Jordan Schoephoerster, Jillian Frisch, Michael Grahek, Chun Wu, Yingwei He, Wei Wang et al.
      Pages 291-300
  7. Back Matter
    Pages 301-302

About this book


Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.  In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC).  Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.


Antigen preservation Cancer Digital imaging High-throughput data Neuroscience Novel assays ST-IHC Stem cell research

Editors and affiliations

  • Alexander E. Kalyuzhny
    • 1
  1. 1.R & D Systems, Inc.MinneapolisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-023-2
  • Online ISBN 978-1-61779-024-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site