Plant Immunity

Methods and Protocols

  • John M. McDowell

Part of the Methods in Molecular Biology book series (MIMB, volume 712)

Table of contents

  1. Front Matter
    Pages i-xv
  2. James M. Elmore, Gitta Coaker
    Pages 31-44
  3. Xue-Cheng Zhang, Walter Gassmann
    Pages 69-77
  4. Ann-Maree Catanzariti, Rohit Mago, Jeff Ellis, Peter Dodds
    Pages 79-87
  5. Edgar Huitema, Matthew Smoker, Sophien Kamoun
    Pages 129-135
  6. John M. McDowell, Troy Hoff, Ryan G. Anderson, Daniel Deegan
    Pages 137-151
  7. Petra C. Boevink, Paul R. J. Birch, Stephen C. Whisson
    Pages 195-209
  8. Eric Kemen, Kurt Mendgen, Ralf T. Voegele
    Pages 211-225
  9. Nagabhushana Ithal, Melissa G. Mitchum
    Pages 227-240

About this book

Introduction

A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes.  In  Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens.  These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity.  Written in the highly successful  Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Keywords

Disease control Immune signaling Laser microdissection Molecular mechanisms Pathogen effector proteins Pathosystems Plant-microbe interactions

Editors and affiliations

  • John M. McDowell
    • 1
  1. 1., Plant Pathology, Physiol., & Weed Sci.Virginia TechBlacksburgUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61737-998-7
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-997-0
  • Online ISBN 978-1-61737-998-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book