Heterologous Gene Expression in E.coli

Methods and Protocols

  • Thomas C. Evans, Jr.
  • Ming-Qun Xu

Part of the Methods in Molecular Biology book series (MIMB, volume 705)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Raymond J. Peroutka III, Steven J. Orcutt, James E. Strickler, Tauseef R. Butt
    Pages 15-30
  3. Adam C. Fisher, Mark A. Rocco, Matthew P. DeLisa
    Pages 53-67
  4. Quan Bai, Xindu Geng
    Pages 69-85
  5. Inca Ghosh, Nancy Considine, Elissa Maunus, Luo Sun, Aihua Zhang, John Buswell et al.
    Pages 87-107
  6. Jonathan P. Richardson, Derek Macmillan
    Pages 151-174
  7. Orsolya Király, Lan Guan, Miklós Sahin-Tóth
    Pages 175-194
  8. Jun-ichi Nagao, Kouki Shioya, Yoshitaka Harada, Ken-ichi Okuda, Takeshi Zendo, Jiro Nakayama et al.
    Pages 225-236
  9. Akira Kanno, Takeaki Ozawa, Yoshio Umezawa
    Pages 251-258
  10. Aihua Zhang, Luo Sun, John Buswell, Nancy Considine, Inca Ghosh, Anastasiya Masharina et al.
    Pages 295-307
  11. Back Matter
    Pages 309-310

About this book

Introduction

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

Keywords

Amino acid residues or moieties Binding partners Escherichia coli Expression hosts Membrane proteins Post-translational modifications Protein expression Recombinant DNA Recombinant proteins

Editors and affiliations

  • Thomas C. Evans, Jr.
    • 1
  • Ming-Qun Xu
    • 2
  1. 1., DNA Enzymes DivisionNew England Biolabs, Inc.IpswichUSA
  2. 2., Chemical Biology DivisionNew England Biolabs, Inc.IpswichUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61737-967-3
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-966-6
  • Online ISBN 978-1-61737-967-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book