Nitric Oxide

Methods and Protocols

  • Helen O. McCarthy
  • Jonathan A. Coulter

Part of the Methods in Molecular Biology book series (MIMB, volume 704)

Table of contents

  1. Front Matter
    Pages i-xi
  2. David G. Hirst, Tracy Robson
    Pages 1-13
  3. Detection and Quantification of Nitric Oxide

    1. Front Matter
      Pages 15-15
    2. Yong Chool Boo, Gyeong In Mun, Sarah L. Tressel, Hanjoong Jo
      Pages 81-89
    3. Marcela A. Segundo, Luís M. Magalhães, Joana P.N. Ribeiro, Marlene Lúcio, Salette Reis
      Pages 91-104
    4. David N. Silverman, Chingkuang Tu
      Pages 105-114
    5. Angela Elia, Pietro Mario Lugarà, Cinzia Di Franco, Vincenzo Spagnolo
      Pages 115-133
  4. Nitric Oxide Generation

    1. Front Matter
      Pages 151-151
    2. Sean O. Hynes, Cillian McCabe, Timothy O’Brien
      Pages 153-168
    3. George Han, Adam J. Friedman, Joel M. Friedman
      Pages 187-195
    4. Beihui Liu, James Hewinson, Haibo Xu, Francisco Montero, Carmen R. Sunico, Federico Portillo et al.
      Pages 197-223
  5. Back Matter
    Pages 225-226

About this book


The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.


Biofilms Detection and quantification In vivo generation Nanoparticles Nitrate Physiological, pathological responses Viral, shRNA delivery systems

Editors and affiliations

  • Helen O. McCarthy
    • 1
  • Jonathan A. Coulter
    • 2
  1. 1.School of PharmacyQueen's University, BelfastBelfastUnited Kingdom
  2. 2.School of PharmacyQueen's University, BelfastBelfastUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-963-5
  • Online ISBN 978-1-61737-964-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site