Transgenic Mouse Methods and Protocols

  • Marten H. Hofker
  • Jan van Deursen

Part of the Methods in Molecular Biology book series (MIMB, volume 693)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Marion J. J. Gijbels, Menno P. J. de Winther
    Pages 75-87
  3. Bart van de Sluis, Jan Willem Voncken
    Pages 89-101
  4. Thomas L. Saunders
    Pages 103-115
  5. Terunaga Nakagawa, Casper C. Hoogenraad
    Pages 117-142
  6. Janine van Ree, Wei Zhou, Ming Li, Jan M. van Deursen
    Pages 143-162
  7. Liviu A. Malureanu
    Pages 181-203
  8. Roland H. Friedel, Wolfgang Wurst, Benedikt Wefers, Ralf Kühn
    Pages 205-231
  9. Darren J. Baker
    Pages 233-244
  10. Chunhong Liu, Paul F. Szurek, Y. Eugene Yu
    Pages 245-256
  11. Anton J. M. Roebroek, Philip L. S. M. Gordts, Sara Reekmans
    Pages 257-275
  12. Anton J. M. Roebroek, Philip L. S. M. Gordts, Sara Reekmans
    Pages 277-281
  13. Kyoji Horie, George Gaitanaris, Alexander Gragerov
    Pages 283-294
  14. Menno P. J. de Winther, Peter Heeringa
    Pages 309-320
  15. Ko Willems van Dijk, Kyriakos E. Kypreos, Frits J. Fallaux, Jurre Hageman
    Pages 321-343
  16. Back Matter
    Pages 345-352

About this book


The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.


Gene function Genotype-phenotype relationships Knockout mice Transgenic mice Transgenic mouse models

Editors and affiliations

  • Marten H. Hofker
    • 1
  • Jan van Deursen
    • 2
  1. 1.Dept. Pathology & Medical Biology, Medical Biology SectionUniversity Medical Center GroningenGroningenNetherlands
  2. 2.Mayo ClinicRochesterUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-973-4
  • Online ISBN 978-1-60761-974-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site