Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

Methods and Protocols

  • Nicole I. Nieden

Part of the Methods in Molecular Biology book series (MIMB, volume 690)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Sabrina Lin, Prue Talbot
    Pages 31-56
  3. Michael D. O’Connor, Melanie D. Kardel, Connie J. Eaves
    Pages 67-80
  4. Helen Spencer, Maria Keramari, Christopher M. Ward
    Pages 81-94
  5. Parinya Noisa, Alai Urrutikoetxea-Uriguen, Wei Cui
    Pages 95-106
  6. Stephanie N. Rushing, Anthony W. Herren, Deborah K. Lieu, Ronald A. Li
    Pages 107-120
  7. Ana Fernandes-Platzgummer, Maria Margarida Diogo, Cláudia Lobato da Silva, Joaquim M. S. Cabral
    Pages 121-134
  8. Susanne Trettner, Alexander Seeliger, Nicole I. zur Nieden
    Pages 135-149
  9. Dawn P. Spelke, Daniel Ortmann, Ali Khademhosseini, Lino Ferreira, Jeffrey M. Karp
    Pages 151-162
  10. Motokazu Tsuneto, Toshiyuki Yamane, Shin-Ichi Hayashi
    Pages 239-253
  11. Lesley A. Davis, Anke Dienelt, Nicole I. zur Nieden
    Pages 255-272
  12. Nobuyuki Udagawa, Teruhito Yamashita, Yasuhiro Kobayashi, Naoyuki Takahashi
    Pages 273-284
  13. Boyangzi Li, Haiying Liu, Zhenqing Zhang, Hope E. Stansfield, Jonathan S. Dordick, Robert J. Linhardt
    Pages 285-300
  14. Back Matter
    Pages 333-336

About this book


Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.


Controllable bioreactor processes Degenerative cartilage & bone diseases ESCs Osteoblasts, osteoclasts, chondrocytes Osteodegenerative Regenerative medicine Skeletal cell types Skeletal tissue repair Skeletogenesis Tissue engineering

Editors and affiliations

  • Nicole I. Nieden
    • 1
  1. 1.Dept. of Cell Biology & NeuroscienceUniversity of California at RiversideRiversideUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-961-1
  • Online ISBN 978-1-60761-962-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site