Light Microscopy

Methods and Protocols

  • Hélio Chiarini-Garcia
  • Rossana C. N. Melo

Part of the Methods in Molecular Biology book series (MIMB, volume 689)

Table of contents

  1. Front Matter
    Pages i-x
  2. Bright-Field Microscopy Applications

    1. Front Matter
      Pages 1-1
    2. Gerluza Aparecida Borges Silva, Adriana Moreira, José Bento Alves
      Pages 19-36
    3. Élder Antônio Sousa Paiva, Sheila Zambello de Pinho, Denise Maria Trombert Oliveira
      Pages 37-49
    4. Carlos Roberto Carvalho, Wellington Ronildo Clarindo, Isabella Santiago Abreu
      Pages 51-68
    5. Daniela L. Fabrino, Grazielle A. Ribeiro, Lívia Teixeira, Rossana C.N. Melo
      Pages 69-80
    6. Vanessa Pinho, Fernanda Matos Coelho, Gustavo Batista Menezes, Denise Carmona Cara
      Pages 81-90
  3. Fluorescence Microscopy Applications

    1. Front Matter
      Pages 91-91
    2. Ionita C. Ghiran
      Pages 93-136
    3. Rossana C.N. Melo, Heloisa D’Ávila, Patricia T. Bozza, Peter F. Weller
      Pages 149-161
    4. Silvia Guatimosim, Cristina Guatimosim, Long-Sheng Song
      Pages 205-214
    5. Maria Carolina S. Soares, Lúcia M. Lobão, Luciana O. Vidal, Natália P. Noyma, Nathan O. Barros, Simone J. Cardoso et al.
      Pages 215-227
  4. Back Matter
    Pages 241-244

About this book


Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.


Bright-field microscopy Microscope instrumentation Plastic resin embedding methods Sample preparation Staining procedures

Editors and affiliations

  • Hélio Chiarini-Garcia
    • 1
  • Rossana C. N. Melo
    • 2
  1. 1.Laboratory of Structural Biology &, ReproductionFederal University of Minas GeraisBelo HorizonteBrazil
  2. 2.Laboratory of Cellular Biology, Department of Biology, ICBFederal University of Juiz de ForaJuiz de Fora, MGBrazil

Bibliographic information

  • DOI
  • Copyright Information Springer Science + Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-949-9
  • Online ISBN 978-1-60761-950-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site