The Blood-Brain and Other Neural Barriers

Reviews and Protocols

  • Sukriti Nag

Part of the Methods in Molecular Biology book series (MIMB, volume 686)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Biology of the Barriers

    1. Front Matter
      Pages 1-1
    2. Paula Dore-Duffy, Kristen Cleary
      Pages 49-68
    3. Sukriti Nag
      Pages 69-100
    4. Conrad E. Johanson, Edward G. Stopa, Paul N. McMillan
      Pages 101-131
    5. E. Aaron Runkle, David A. Antonetti
      Pages 133-148
    6. Ananda Weerasuriya, Andrew P. Mizisin
      Pages 149-173
  3. Imaging the Barriers

    1. Front Matter
      Pages 175-175
    2. Janet L. Manias, Anish Kapadia, Sukriti Nag
      Pages 177-192
    3. Tavarekere N. Nagaraja, Robert A. Knight, James R. Ewing, Kishor Karki, Vijaya Nagesh, Joseph D. Fenstermacher
      Pages 193-212
    4. Daniel C. Anthony, Nicola R. Sibson, Martina A. McAteer, Ben Davis, Robin P. Choudhury
      Pages 213-227
    5. Norman R. Saunders, C. Joakim Ek, Mark D. Habgood, Pia Johansson, Shane Liddelow, Katarzyna M. Dziegielewska
      Pages 247-265
  4. Molecular Techniques to Study the Blood–Brain Barrier

    1. Front Matter
      Pages 273-273
    2. Romain Cayrol, Arsalan S. Haqqani, Igal Ifergan, Aurore Dodelet-Devillers, Alexandre Prat
      Pages 275-295
    3. Nivetha Murugesan, Jennifer A. Macdonald, Qiaozhan Lu, Shiaw-Lin Wu, William S. Hancock, Joel S. Pachter
      Pages 297-311
    4. Gary N. Y. Chan, Reina Bendayan
      Pages 313-336
    5. Arsalan S. Haqqani, Jennifer J. Hill, James Mullen, Danica B. Stanimirovic
      Pages 337-353
  5. Models to Study the Barriers

    1. Front Matter
      Pages 355-355
    2. Robert L. Pinsonneault, Nasima Mayer, Fahima Mayer, Nebiyu Tegegn, Roland J. Bainton
      Pages 357-369
    3. Brian P. Eliceiri, Ana Maria Gonzalez, Andrew Baird
      Pages 371-378
    4. Gokulan Thanabalasundaram, Jehad El-Gindi, Mira Lischper, Hans-Joachim Galla
      Pages 379-399
  6. Delivery of Therapeutic Agents Across the Barriers

    1. Front Matter
      Pages 427-427
    2. Marie Blanchette, David Fortin
      Pages 447-463
    3. Umar Iqbal, Abedelnasser Abulrob, Danica B. Stanimirovic
      Pages 465-481
    4. Andrew Baird, Brian P. Eliceiri, Ana Maria Gonzalez, Conrad E. Johanson, Wendy Leadbeater, Edward G. Stopa
      Pages 483-498
  7. Back Matter
    Pages 499-502

About this book


Evolving technologies starting with tracer studies, and more recently with genomics and proteomics, have provided novel information about the molecular properties of cerebral endothelium and astrocytes; however, further studies must be done in animal models of neurological diseases and in humans to get a clearer understanding of the pathogenesis of blood-brain barrier (BBB) breakdown in nervous system diseases. In The Blood-Brain and Other Neural Barriers: Reviews and Protocols, experts in the field present a series of cutting-edge protocols which can be used to study the barriers. Opening with detailed information on components of the neurovascular unit as well as the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, the book continues with meticulous techniques to image the barriers in humans and experimental animals, followed by cutting-edge molecular techniques to study the BBB and novel models to study the barriers, and it concludes with techniques for the delivery of therapeutic agents across the BBB. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, The Blood-Brain and Other Neural Barriers: Reviews and Protocols will aid the research efforts of not only graduate students but also more experienced investigators and support future studies of these vital systems.


BBB breakdown Blood-brain barrier Blood-cerebrospinal barrier Blood-nerve barrier Blood-retinal barrier Brain endothelial cells Cerebral endothelium Pericytes, astrocytes, & neurons Therapeutic agents

Editors and affiliations

  • Sukriti Nag
    • 1
  1. 1.Banting Institute, Dept. Laboratory Med. & PathobiologyUniversity of TorontoTorontoCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-937-6
  • Online ISBN 978-1-60761-938-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site