Cell-Penetrating Peptides

Methods and Protocols

  • Ülo Langel

Part of the Methods in Molecular Biology book series (MIMB, volume 683)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Maria Lindgren, Ülo Langel
      Pages 3-19
    3. Edmond Dupont, Alain Prochiantz, Alain Joliot
      Pages 21-29
  3. Methods to Test Mechanisms of Cell-Penetrating Peptides

    1. Front Matter
      Pages 31-31
    2. Astrid Gräslund, Lena Mäler
      Pages 33-40
    3. Sébastien Deshayes, Karidia Konate, Gudrun Aldrian, Frédéric Heitz, Gilles Divita
      Pages 41-56
    4. Ivo R. Ruttekolk, Wouter P. R. Verdurmen, Yi-Da Chung, Roland Brock
      Pages 69-80
    5. Anders Wittrup, Si-He Zhang, Mattias Belting
      Pages 99-115
    6. Anders Florén, Imre Mäger, Ülo Langel
      Pages 117-128
    7. Reto Sauder, Joachim Seelig, André Ziegler
      Pages 129-155
    8. Caroline Palm-Apergi, Mattias Hällbrink
      Pages 157-164
    9. Helin Räägel, Pille Säälik, Ülo Langel, Margus Pooga
      Pages 165-179
    10. Kärt Padari, Annely Lorents, Eija Jokitalo, Margus Pooga
      Pages 181-193
    11. Per Lundin, Samir EL Andaloussi, Ülo Langel
      Pages 195-205
    12. Tina Holm, Samir EL Andaloussi, Ülo Langel
      Pages 207-217
    13. Peter Guterstam, Samir EL Andaloussi, Ülo Langel
      Pages 219-230
  4. Functionality and Cell-Penetrating Peptides

    1. Front Matter
      Pages 231-231
    2. Henrik J. Johansson, Samir EL Andaloussi, Ülo Langel
      Pages 233-247
    3. Sarah L. Tressel, Georgios Koukos, Boris Tchernychev, Suzanne L. Jacques, Lidija Covic, Athan Kuliopulos
      Pages 259-275
  5. Applications of CPPs in Gene Modulation

    1. Front Matter
      Pages 305-305
    2. Rachida Abes, Andrey A. Arzumanov, Amer F. Saleh, Fatouma Said Hassane, Michael J. Gait, Bernard Lebleu
      Pages 307-320
    3. HaiFang Yin, Hong Moulton, Corinne Betts, Matthew Wood
      Pages 321-338
    4. Caroline Palm-Apergi, Akiko Eguchi, Steven F. Dowdy
      Pages 339-347
    5. Laurence Crombez, Gilles Divita
      Pages 349-360
    6. Samir EL Andaloussi, Taavi Lehto, Per Lundin, Ülo Langel
      Pages 361-373
    7. Franziska Mussbach, Regina Pietrucha, Buerk Schaefer, Siegmund Reissmann
      Pages 375-389
  6. CPP In Vivo and as Future Drugs

    1. Front Matter
      Pages 399-399
    2. Sterghios A. Moschos, Karen G. Spink, Mark A. Lindsay
      Pages 417-429
    3. Rupa Sawant, Vladimir Torchilin
      Pages 431-451
    4. Thomas J. Bell, Emelía Eiríksdóttir, Ülo Langel, James Eberwine
      Pages 473-486
    5. Ulf Tedebark, Anthony Scozzari, Oleg Werbitzky, Daniel Capaldi, Lars Holmberg
      Pages 505-524
    6. Ikuhiko Nakase, Kentaro Kogure, Hideyoshi Harashima, Shiroh Futaki
      Pages 525-533
    7. Randolph M. Johnson, Stephen D. Harrison, Derek Maclean
      Pages 535-551
  7. Back Matter
    Pages 573-586

About this book


In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs.


CPP mechanisms CPP-assisted delivery Cellular uptake Drug development Gene modulation

Editors and affiliations

  • Ülo Langel
    • 1
  1. 1., Department of NeurochemistryStockholm UniversityStockholmSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-918-5
  • Online ISBN 978-1-60761-919-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site