MicroRNA and Cancer

Methods and Protocols

  • Wei Wu

Part of the Methods in Molecular Biology book series (MIMB, volume 676)

Table of contents

  1. Front Matter
    Pages i-x
  2. Reviews

    1. Front Matter
      Pages 1-1
    2. Julia Winter, Sven Diederichs
      Pages 3-22
    3. Anastasis Oulas, Nestoras Karathanasis, Panayiota Poirazi
      Pages 23-41
  3. Protocols

    1. Front Matter
      Pages 71-71
    2. Elena V. Grigorenko, Elen Ortenberg, James Hurley, Andrew Bond, Kevin Munnelly
      Pages 101-110
    3. Keith M. Giles, Andrew Barker, Priscilla M. Zhang, Michael R. Epis, Peter J. Leedman
      Pages 147-163
    4. Hani Choudhry, James W. F. Catto
      Pages 165-184
    5. Zejuan Li, Jianjun Chen
      Pages 185-195
    6. Debora Landi, Roberto Barale, Federica Gemignani, Stefano Landi
      Pages 197-210
    7. Shu-Jen Chen, Hua-Chien Chen
      Pages 225-241
    8. Anastasis Oulas, Panayiota Poirazi
      Pages 243-252
    9. Thomas Streichert, Benjamin Otto, Ulrich Lehmann
      Pages 253-268
  4. Back Matter
    Pages 269-273

About this book


The tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In MicroRNA and Cancer: Methods and Protocols, internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Ranging from the fundamental concepts to practical applications, this book presents: • Overview of microRNA biogenesis, computational prediction of new miRNAs in the cancer genome, and miRNA-based therapeutic approaches for cancer treatment • Detailed experimental protocols in miRNA detection with novel and high-throughput technology, miRNA library cloning, miRNA epigenetic regulation, and miRNA pathway study • Stepwise computational and bioinformatic procedures for miRNA complex networks in cancer genomes with a variety of softwares and programs • Cross-cited notes on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs.


Biogenesis Cancer biomarkers Cancer genome Computational prediction Epigenetic regulation Library construction Regulatory networks miRNA

Editors and affiliations

  • Wei Wu
    • 1
  1. 1., Department of Biological ScienceUniversity of CalgaryCalgaryCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-862-1
  • Online ISBN 978-1-60761-863-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site