Methods and Protocols

  • Wolfgang R. Streit
  • Rolf Daniel

Part of the Methods in Molecular Biology book series (MIMB, volume 668)

Table of contents

  1. Front Matter
    Pages i-xi
  2. José M. Vieites, María-Eugenia Guazzaroni, Ana Beloqui, Peter N. Golyshin, Manuel Ferrer
    Pages 1-37
  3. Nancy Weiland, Carolin Löscher, Rebekka Metzger, Ruth Schmitz
    Pages 51-65
  4. Yin Chen, Josh D. Neufeld, Marc G. Dumont, Michael W. Friedrich, J. Colin Murrell
    Pages 67-75
  5. Margaret Wexler, Andrew W. B. Johnston
    Pages 77-96
  6. Kristof Vrancken, Lieve Van Mellaert, Jozef Anné
    Pages 97-107
  7. Sonja Christina Troeschel, Thomas Drepper, Christian Leggewie, Wolfgang R. Streit, Karl-Erich Jaeger
    Pages 117-139
  8. Dominique Böttcher, Marlen Schmidt, Uwe T. Bornscheuer
    Pages 169-176
  9. Nele Ilmberger, Wolfgang R. Streit
    Pages 177-188
  10. Manuel Ferrer, Ana Beloqui, Peter N. Golyshin
    Pages 189-202
  11. Phil M. Oger, Stéphane Uroz
    Pages 203-217
  12. Massimo Marzorati, Annalisa Balloi, Francesca De Ferra, Daniele Daffonchio
    Pages 219-234
  13. Ricardo F. Nordeste, Maria A. Trainer, Trevor C. Charles
    Pages 235-246
  14. Mindy G. Brown, Elizabeth H. Mitchell, David L. Balkwill
    Pages 265-271
  15. José E. González-Pastor, Salvador Mirete
    Pages 273-285
  16. Tracy Meiring, Inonge Mulako, Marla I. Tuffin, Quinton Meyer, Donald A. Cowan
    Pages 287-297
  17. Soo-Je Park, Jong-Chan Chae, Sung-Keun Rhee
    Pages 313-324
  18. Back Matter
    Pages 339-341

About this book


Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries. In Metagenomics: Methods and Protocols, expert researches provide an overview and introduction to basic methods commonly used in laboratories that have a strong background in microbial metagenomics. The book attempts to address all of the working steps involved in this crucial field, beginning with DNA isolation from soils and marine samples and continuing with the construction and screening of libraries, along with key advise involving bioinformatic tools available to analyze large metagenomic sequence data sets. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metagenomics: Methods and Protocols serves as a very complete guide to available screening protocols for all major biocatalysts in order to allow for the easy setup of these screens in any microbiology lab.


Biodiversity Bioindustrial drug molecules DNA Ecology Metabolic profiling Metagenomics Microbial DNA Promoter Uncultivated microorganisms gene expression genes hybridization metagenome microbiology microorganism

Editors and affiliations

  • Wolfgang R. Streit
    • 1
  • Rolf Daniel
    • 2
  1. 1.Biocenter Klein Flottbek, Microbiology & BiotechnologyUniversity of HamburgHamburgGermany
  2. 2.Institute of Microbiology and Genetics, Dept. of Genomic & Applied MicrobiologyGeorg August UniversityGöttingenGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-822-5
  • Online ISBN 978-1-60761-823-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site