Diagnostic Virology Protocols

  • John R. Stephenson
  • Alan Warnes

Part of the Methods in Molecular Biology book series (MIMB, volume 665)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Kanti Pabbaraju, Sallene Wong, Julie D. Fox
    Pages 1-15
  3. A. M. Powers, John T. Roehrig
    Pages 17-38
  4. Robert L. Atmar, Frederick H. Neill, Françoise S. Le Guyader
    Pages 39-50
  5. Nagesh K. Tripathi, Ambuj Shrivastava, Paban K. Dash, Asha M. Jana
    Pages 51-64
  6. Chit Laa Poh, Eng Lee Tan
    Pages 65-77
  7. Peter Karayiannis
    Pages 79-100
  8. Harald H. Kessler, Sophie Heyszl
    Pages 101-121
  9. Patricia Cane
    Pages 123-132
  10. Neil Berry, Carolina Herrera, Martin Cranage
    Pages 133-160
  11. Graham Tipples, Joanne Hiebert
    Pages 183-193
  12. Sean Doyle
    Pages 213-231
  13. Andreas Kurth, Andreas Nitsche
    Pages 257-278
  14. Jim Gray, Miren Iturriza-Gómara
    Pages 325-355
  15. Sofi M. Ibrahim, Mohamed Aitichou, Justin Hardick, Jamie Blow, Monica L. O’Guinn, Connie Schmaljohn
    Pages 357-368
  16. J. S. Malik Peiris, Leo L. M. Poon
    Pages 369-382
  17. Elizabeth B. Kauffman, Mary A. Franke, Susan J. Wong, Laura D. Kramer
    Pages 383-413
  18. Christopher C. Blyth, Robert Booy, Dominic E. Dwyer
    Pages 415-433
  19. Back Matter
    Pages 467-470

About this book


Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.


Mathematical modelling Microfluidics systems Nucleic acid detection Polymerase chain reaction (PCR) Robotic analyzers Viruses

Editors and affiliations

  • John R. Stephenson
    • 1
  • Alan Warnes
    • 2
  1. 1., Research & Development DivisionHealth Protection AgencyLondonUnited Kingdom
  2. 2.Northwick Park Hospital, Research and DevelopmentNorth West London Hospitals NHS TrustHarrowUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-816-4
  • Online ISBN 978-1-60761-817-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site