MicroRNAs and the Immune System

Methods and Protocols

  • Silvia Monticelli

Part of the Methods in Molecular Biology book series (MIMB, volume 667)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Analysis of miRNA Expression: Classic Methods Revisited

  3. High-Throughput Analysis of miRNAs

    1. Front Matter
      Pages 65-65
    2. Haoquan Wu, Joel Neilson, N. Manjunath
      Pages 67-77
    3. Muller Fabbri, Riccardo Spizzo, George A. Calin
      Pages 79-91
    4. Molly F. Thomas, K. Mark Ansel
      Pages 93-111
  4. Functional Analysis of miRNAs in the Immune System: Gain-of-Function

    1. Front Matter
      Pages 127-127
    2. Raquel Malumbres, Izidore S. Lossos
      Pages 129-143
    3. Laura Fontana, Antonio Sorrentino, Cesare Peschle
      Pages 165-176
    4. Virginia G. de Yébenes, Almudena R. Ramiro
      Pages 177-192
    5. Carolyn Sangokoya, Gregory LaMonte, Jen-Tsan Chi
      Pages 193-203
  5. Functional Analysis of miRNAs in the Immune System: Loss-of-Function

    1. Front Matter
      Pages 235-235
    2. Ewa Surdziel, Matthias Eder, Michaela Scherr
      Pages 237-248
  6. miRNA Post-Transcriptional Modifications and Mechanisms of Action

    1. Front Matter
      Pages 249-249
    2. Mirko Cevec, Janez Plavec
      Pages 251-265
    3. Dylan E. Dupuis, Stefan Maas
      Pages 267-279
  7. Bioinformatic Analysis and Target Prediction

    1. Front Matter
      Pages 281-281
    2. Preethi H. Gunaratne, Chad J. Creighton, Michael Watson, Jayantha B. Tennakoon
      Pages 297-315
  8. miRNA and Viruses

    1. Front Matter
      Pages 317-317
  9. Back Matter
    Pages 327-330

About this book


In recent years, the critical role of microRNAs has been revealed within the biology of cells that constitute the immune system. In MicroRNAs and the Immune System: Methods and Protocols, expert researchers explore the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as high-throughput profiling through cloning, deep sequencing, and microarrays. Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response. Tools are also provided to help navigate bioinformatics databases on miRNAs and their targets. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and innovative, MicroRNAs and the Immune System: Methods and Protocols is an essential handbook for immunologists, biochemists, and molecular biologists.


Bioinformatics databases Deep sequencing Fluoreszenz in situ-Hybridisierung Immune response MiRNA expression Microarray Microarrays PCR Regulation Viruses bioinformatics gene expression miRNAs molecular biology transcription

Editors and affiliations

  • Silvia Monticelli
    • 1
  1. 1.Institute for Research in BiomedicineBellinzonaSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science + Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-810-2
  • Online ISBN 978-1-60761-811-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site