Membrane Protein Structure Determination

Methods and Protocols

  • Jean-Jacques Lacapère

Part of the Methods in Molecular Biology book series (MIMB, volume 654)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Membrane Protein Purification

    1. Front Matter
      Pages 1-1
    2. Mariano A. Ostuni, Soria Iatmanen, David Teboul, Jean-Claude Robert, Jean-Jacques Lacapère
      Pages 3-18
    3. Bertrand Arnou, Cécile Dahout-Gonzalez, Ludovic Pelosi, Guy J.-M. Lauquin, Gérard Brandolin, Véronique Trézéguet
      Pages 19-28
    4. Jean-Claude Robert, Jean-Jacques Lacapère
      Pages 29-45
    5. Alexandre Pozza, José M. Pérez-Victoria, Attilio Di Pietro
      Pages 47-75
  3. X-Ray Crystallography

    1. Front Matter
      Pages 77-77
    2. Aurélien Deniaud, Ekaterina Moiseeva, Valentin Gordeliy, Eva Pebay-Peyroula
      Pages 79-103
    3. Hugues Nury, Iulia Blesneac, Stephanie Ravaud, Eva Pebay-Peyroula
      Pages 105-117
  4. Electron Microscopy

    1. Front Matter
      Pages 169-169
    2. David L. Stokes, William J. Rice, Minghui Hu, Changki Kim, Iban Ubarretxena-Belandia
      Pages 187-205
    3. Sylvain Trépout, Jean-Christophe Taveau, Olivier Lambert
      Pages 207-220
    4. Gabriel Péranzi, Cedric Messaoudi, Leeyah Issop, Jean-Jacques Lacapère
      Pages 221-235
    5. Konrad Hinsen, Edward Beaumont, Bertrand Fournier, Jean-Jacques Lacapère
      Pages 237-258
  5. Nuclear Magnetic Resonance

    1. Front Matter
      Pages 259-259
    2. Gianluigi Veglia, Kim N. Ha, Lei Shi, Raffaello Verardi, Nathaniel J. Traaseth
      Pages 303-319
    3. Axelle Grélard, Cécile Loudet, Anna Diller, Erick J. Dufourc
      Pages 341-359
  6. Molecular Modelling

    1. Front Matter
      Pages 361-361
    2. Alexandre G. de Brevern
      Pages 387-401
    3. Christian Kandt, Luca Monticelli
      Pages 423-440
    4. Irina G. Tikhonova, Daniel Fourmy
      Pages 441-454
  7. Back Matter
    Pages 455-459

About this book


Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.


BCG Electron microscopy Membrane protein purification Molecular modelling Nuclear magnetic resonance - NMR PCR Termination X-ray crystallography cell biology cellular processes liposomes molecular biology protein family protein structure tertiary structure

Editors and affiliations

  • Jean-Jacques Lacapère
    • 1
  1. 1., Université Paris Diderot - Paris 7INSERM U773/CRB3ParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-761-7
  • Online ISBN 978-1-60761-762-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site