Mass Spectrometry Imaging

Principles and Protocols

  • Stanislav S. Rubakhin
  • Jonathan V. Sweedler

Part of the Methods in Molecular Biology book series (MIMB, volume 656)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introductory Reviews and Tutorials

    1. Front Matter
      Pages 1-1
    2. Sarah A. Schwartz, Richard M. Caprioli
      Pages 3-19
    3. Stanislav S. Rubakhin, Jonathan V. Sweedler
      Pages 21-49
  3. Protocols for MS Imaging of Distribution of Small Molecules Including Metabolites and Pharmaceuticals

    1. Front Matter
      Pages 83-83
    2. Michael L. Heien, Paul D. Piehowski, Nicholas Winograd, Andrew G. Ewing
      Pages 85-97
    3. Yanfeng Chen, Ying Liu, Jeremy Allegood, Elaine Wang, Begoña Cachón-González, Timothy M. Cox et al.
      Pages 131-146
    4. Yunsheng Hsieh, Fangbiao Li, Walter A. Korfmacher
      Pages 147-158
    5. A.F. Maarten Altelaar, Sander R. Piersma
      Pages 197-208
    6. Timothy J. Garrett, Richard A. Yost
      Pages 209-230
    7. Vilmos Kertesz, Gary J. Van Berkel
      Pages 231-241
    8. Elena S.F. Berman, Susan L. Fortson, Kristen S. Kulp
      Pages 253-265
    9. Ligang Wu, James S. Felton, Kuang Jen J. Wu
      Pages 267-281
  4. Protocols for MS Imaging of Distribution of Peptides and Proteins

    1. Front Matter
      Pages 283-283
    2. Michelle L. Reyzer, Pierre Chaurand, Peggi M. Angel, Richard M. Caprioli
      Pages 285-301
    3. Maxence Wisztorski, Julien Franck, Michel Salzet, Isabelle Fournier
      Pages 303-322
    4. Julien Franck, Mohamed El Ayed, Maxence Wisztorski, Michel Salzet, Isabelle Fournier
      Pages 323-338
    5. Jonathan Stauber, Mohamed El Ayed, Maxence Wisztorski, Michel Salzet, Isabelle Fournier
      Pages 339-361
    6. John A. McLean, Larissa S. Fenn, Jeffrey R. Enders
      Pages 363-383
    7. Sören-Oliver Deininger, Michael Becker, Detlev Suckau
      Pages 385-403
    8. Brendan Prideaux, Dieter Staab, Markus Stoeckli
      Pages 405-413
    9. Nathalie Y.R. Agar, Jane-Marie Kowalski, Paul J. Kowalski, John H. Wong, Jeffrey N. Agar
      Pages 415-431
    10. Peter D.E.M. Verhaert, Martijn W.H. Pinkse, Kerstin Strupat, Maria C. Prieto Conaway
      Pages 433-449
    11. Ruibing Chen, Stephanie S. Cape, Robert M. Sturm, Lingjun Li
      Pages 451-463
    12. Tyler A. Zimmerman, Stanislav S. Rubakhin, Jonathan V. Sweedler
      Pages 465-479
  5. Back Matter
    Pages 481-487

About this book


Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting. Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.


Analytes Distribution of peptides and proteins Distributions MS imaging Mass Spectrometry Metabolites and pharmaceuticals preparation tissue

Editors and affiliations

  • Stanislav S. Rubakhin
    • 1
  • Jonathan V. Sweedler
    • 2
  1. 1., Beckman InstituteUniv. of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2., Department of ChemistryUniv. of Illinois at Urbana-ChampaignUrbanaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-745-7
  • Online ISBN 978-1-60761-746-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site