Transcription Factors

Methods and Protocols

  • Paul J. Higgins

Part of the Methods in Molecular Biology book series (MIMB, volume 647)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Transcription Factor Trafficking and Post-translational Modifications: Overviews of Mechanisms

  3. Cytoplasmic-Nuclear Trafficking of Transcription Factors

    1. Front Matter
      Pages 76-76
    2. Sayyed K. Zaidi, Ricardo F. Medina, Shirwin M. Pockwinse, Rachit Bakshi, Krishna P. Kota, Syed A. Ali et al.
      Pages 77-93
    3. Richard Y. M. Ma, Tommy H. K. Tong, Wai Ying Leung, Kwok-Ming Yao
      Pages 113-123
    4. Fangyan Dai, Xueyan Duan, Yao-Yun Liang, Xia Lin, Xin-Hua Feng
      Pages 125-137
    5. Kerry-Ann Nakrieko, Iordanka A. Ivanova, Lina Dagnino
      Pages 161-170
    6. Tina S. Mehta, Farah Monzur, Jihe Zhao
      Pages 171-186
  4. Post-translational Modifications and Impact on Function

    1. Front Matter
      Pages 214-214
    2. Kapil Bharti, Julien Debbache, Xin Wang, Heinz Arnheiter
      Pages 237-250
    3. Sébastien Lalevée, Christine Ferry, Cécile Rochette-Egly
      Pages 251-266
    4. Dimple Notani, Amita S. Limaye, P. Pavan Kumar, Sanjeev Galande
      Pages 317-335
  5. Protocols for Optimization of Functional Assays

  6. Back Matter
    Pages 407-412

About this book


In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Transcription Factors: Methods and Protocols compiles the latest techniques for elucidating controls on transcription factor intracellular localization and activity, and consequently is unlike any other methods-based text on transcriptional regulation today. 


DNA Intracellular trafficking Kinase targeting Nuclear translocation Phosphorylation Post-translational modifications Protein degradation Regulation Termination Translation gene expression genes molecular control molecular mechanisms post-translational modification

Editors and affiliations

  • Paul J. Higgins
    • 1
  1. 1.Ctr. Cell Biology & Cancer ResearchAlbany Medical CollegeAlbanyUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-737-2
  • Online ISBN 978-1-60761-738-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site