The Urinary Proteome

Methods and Protocols

  • Alex J. Rai

Part of the Methods in Molecular Biology book series (MIMB, volume 641)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Germán Echeverry, Glen L. Hortin, Alex J. Rai
    Pages 1-12
  3. Aude L. Foucher, David R. Craft, Craig A. Gelfand
    Pages 27-45
  4. Georg Martin Fiedler, Uta Ceglarek, Alexander Leichtle, Joachim Thiery
    Pages 47-63
  5. Supachok Sinchaikul, Payungsak Tantipaiboonwong, Supawadee Sriyam, Ching Tzao, Suree Phutrakul, Shui-Tein Chen
    Pages 65-88
  6. Patricia A. Gonzales, Hua Zhou, Trairak Pisitkun, Nam Sun Wang, Robert A. Star, Mark A. Knepper et al.
    Pages 89-99
  7. Laurence D. Parnell, Christine M. E. Schueller
    Pages 101-122
  8. Walter Hofmann, Cornelia Sedlmeir-Hofmann, Miroslav Ivandic´, Dagmar Ruth, Peter Luppa
    Pages 123-142
  9. Jeffrey S. Morris, Keith A. Baggerly, Howard B. Gutstein, Kevin R. Coombes
    Pages 143-166
  10. Peter B. Luppa, Jochen Metzger, Heike Schneider
    Pages 193-221
  11. Ayelet Snow, David Gozal, Roland Valdes Jr., Saeed A. Jortani
    Pages 223-241
  12. Barry Schweitzer, Lihao Meng, Dawn Mattoon, Alex J. Rai
    Pages 243-252
  13. Kris E. Gaston, H. Barton Grossman
    Pages 303-323
  14. Back Matter
    Pages 359-361

About this book


Urine is one of the most easily accessible biological samples, and it provides a treasure trove of molecules essential for clinical diagnostics. In The Urinary Proteome: Methods and Protocols, expert researchers review briefly the classical urine tests that are performed in the clinical laboratory and then delve into the state of the art methods for proteomic analysis using urine specimens. Featuring the most recent advances in sample preparation, data analysis, and methods and applications, the book contains a multitude of detailed methods, including procedural details for the identification and characterization of urine biomarkers, which hold great potential for the diagnosis and treatment of many different disease conditions. Written in the highly successful Methods in Molecular Biology™ series format, chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.

Authoritative and systematic, The Urinary Proteome: Methods and Protocols is an ideal guide for scientific researchers, clinical laboratorians, clinical and translational scientists, and others interested in the vital study of proteomics and biomarker discovery.


Clinical diagnostics Data analysis Microarray Proteomics Sample preparation Translation Urine biomarkers bioinformatics molecular biology

Editors and affiliations

  • Alex J. Rai
    • 1
  1. 1.Medical Center, Dept. Pathology & Cell BiologyColumbia UniversityNew YorkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-710-5
  • Online ISBN 978-1-60761-711-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site