Plant Stress Tolerance

Methods and Protocols

  • Ramanjulu Sunkar

Part of the Methods in Molecular Biology book series (MIMB, volume 639)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Reviews and Overview Chapters

    1. Front Matter
      Pages 1-1
    2. Melvin J. Oliver, John C. Cushman, Karen L. Koster
      Pages 3-24
    3. Viswanathan Chinnusamy, Jian-Kang Zhu, Ramanjulu Sunkar
      Pages 39-55
    4. Nese Sreenivasulu, Ramanjulu Sunkar, Ulrich Wobus, Marc Strickert
      Pages 71-93
    5. Ananda Mustafiz, Khirod K. Sahoo, Sneh L. Singla-Pareek, Sudhir K. Sopory
      Pages 95-118
  3. Genome-Wide Approaches for Identification of Stress-Regulated Genes, Proteins, and Small RNAs

    1. Front Matter
      Pages 119-119
    2. Csaba Papdi, Jeffrey Leung, Mary Prathiba Joseph, Imma Perez Salamó, László Szabados
      Pages 121-139
    3. Akihiro Matsui, Junko Ishida, Taeko Morosawa, Masanori Okamoto, Jong-Myong Kim, Yukio Kurihara et al.
      Pages 141-155
    4. Muthappa Senthil-Kumar, Makarla Udayakumar, Kirankumar S. Mysore
      Pages 193-206
    5. Meenakumari Muthuramalingam, Karl-Josef Dietz, Elke Ströher
      Pages 219-238
    6. Jose Luis Reyes, Catalina Arenas-Huertero, Ramanjulu Sunkar
      Pages 239-251
    7. Xiaoyun Jia, Venugopal Mendu, Guiliang Tang
      Pages 253-269
  4. Antioxidant Enzymes and Metabolites

  5. Osmotic Adjustment and Ion Measurements

    1. Front Matter
      Pages 299-299
    2. Edit Ábrahám, Cecile Hourton-Cabassa, László Erdei, László Szabados
      Pages 317-331
    3. Elodie Parre, Jacques de Virville, Françoise Cochet, Anne-Sophie Leprince, Luc Richard, Delphine Lefebvre-De Vos et al.
      Pages 333-340
    4. Rana Munns, Patricia A. Wallace, Natasha L. Teakle, Timothy D. Colmer
      Pages 371-382
  6. Back Matter
    Pages 383-386

About this book


A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical.  In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results.  Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.


Liana Protein Termination bioinformatics genes hybridization molecular biology regulation

Editors and affiliations

  • Ramanjulu Sunkar
    • 1
  1. 1.246 NRC-D, Department of Biochemistry and MolecularOklahoma State UniversityStillwaterUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-701-3
  • Online ISBN 978-1-60761-702-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site