Hepatocytes

Methods and Protocols

  • Patrick Maurel

Part of the Methods in Molecular Biology book series (MIMB, volume 640)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Christiane Guguen-Guillouzo, Andre Guillouzo
    Pages 1-40
  3. Anne Weber, Thomas Touboul, Sylvie Mainot, Julie Branger, Dominique Mahieu-Caputo
    Pages 41-55
  4. Robin D. Hughes, Ragai R. Mitry, Sharon C. Lehec
    Pages 107-113
  5. Katy M. Olsavsky Goyak, Elizabeth M. Laurenzana, Curtis J. Omiecinski
    Pages 115-138
  6. Robert E. Schwartz, Catherine Verfaillie
    Pages 167-179
  7. Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, Naoya Kobayashi
    Pages 181-236
  8. Sabine Gerbal-Chaloin, Cédric Duret, Edith Raulet, Francis Navarro, Pierre Blanc, Jeanne Ramos et al.
    Pages 247-260
  9. Chia-Ning Shen, David Tosh
    Pages 273-280
  10. Sylvie Klieber, François Torreilles, François Guillou, Gérard Fabre
    Pages 295-308
  11. Sylvie Fournel-Gigleux, Michael W.H. Coughtrie, Mohamed Ouzzine, Jacques Magdalou
    Pages 309-326
  12. Kazuya Maeda, Yuichi Sugiyama
    Pages 327-353
  13. Vincent Croixmarie, Thierry Umbdenstock, Olivier Cloarec, Amélie Moreau, Jean-Marc Pascussi, Yannick Parmentier et al.
    Pages 355-374

About this book

Introduction

Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular Biology™ series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

Keywords

Cryopreservation Detoxication Drug/xenobiotic metabolism and transport Isolation, culture, and differentiation Liver physiopathology Stem cells Transplantation cell biology cells tissue

Editors and affiliations

  • Patrick Maurel
    • 1
  1. 1.INSERM U632, Labo. Physiopathologie HépatiqueUniversité Montpellier IMontpellierFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-688-7
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-687-0
  • Online ISBN 978-1-60761-688-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book