Antimicrobial Peptides

Methods and Protocols

  • Andrea Giuliani
  • Andrea C. Rinaldi

Part of the Methods in Molecular Biology book series (MIMB, volume 618)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Isolation, Purification, Design, and Synthesis of Antimicrobial Peptides

  3. Analysis, Properties, and Mechanisms of Antimicrobial Peptides

    1. Front Matter
      Pages 135-135
    2. Andrea Giuliani, Giovanna Pirri, Andrea C. Rinaldi
      Pages 137-154
    3. Paulo F. Almeida, Antje Pokorny
      Pages 155-169
    4. Nathaniel P. Chongsiriwatana, Annelise E. Barron
      Pages 171-182
    5. Stephan L. Grage, Sergii Afonin, Anne S. Ulrich
      Pages 183-207
    6. Christopher Aisenbrey, Philippe Bertani, Burkhard Bechinger
      Pages 209-233
    7. Ludovica Marcellini, Maria Giammatteo, Pierpaolo Aimola, Maria Luisa Mangoni
      Pages 249-266
    8. Allison Langham, Yiannis N. Kaznessis
      Pages 267-285
  4. Antimicrobial Peptides as Therapeutic Agents

    1. Front Matter
      Pages 301-301
    2. Lijuan Zhang, Timothy J. Falla
      Pages 303-327
    3. Håvard Jenssen, Robert E. W. Hancock
      Pages 329-347
    4. Evelina Rubinchik, Christopher Pasetka
      Pages 349-358
    5. Evelina Rubinchik, Christopher Pasetka
      Pages 359-369
    6. Gill Diamond, Sunghan Yim, Isaura Rigo, Laura McMahon
      Pages 371-382
    7. Juan Román Luque-Ortega, Luis Rivas
      Pages 393-420
  5. Back Matter
    Pages 421-424

About this book


The action of antimicrobial peptides (AMPs), ranging from direct killing of invading pathogens to immune response modulation and other complex biological responses, has stimulated research and clinical interest for more than two decades, but the area is still burgeoning due to emerging discoveries in the functions, roles, and regulation of AMPs, thus making the study of antimicrobial peptides a multi-disciplinary and rapidly evolving field. In Antimicrobial Peptides: Methods and Protocols, leading investigators present a broad, up-to-date collection of current research and experimental methods for the isolation, characterization, production, and optimization of antimicrobial peptides. Additional chapters detail methodologies in several microscopy techniques, high-throughput screening, QSAR modeling, and computer-aided design used to study these compounds, while key review articles survey potential medical applications of antimicrobial peptides as innovative anti-infective and immunomodulatory agents, as well as emerging discoveries in their function, regulation, and roles in innate immunity. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and wide-ranging in its applications, Antimicrobial Peptides: Methods and Protocols provides both an authoritative guide for lab work on AMPs or related substances and a useful collection of thought stimuli to inspire further scientific endeavours in a wide array of vital fields.


Escherichia coli antibiotics bacteria biology electron microscopy immune response immune system immunity infection innate immunity membrane microscopy molecular biology peptides research

Editors and affiliations

  • Andrea Giuliani
    • 1
  • Andrea C. Rinaldi
    • 2
  1. 1.SpiderBiotech S.r.l.Colleretto GiacosaItaly
  2. 2.Dipto. Scienze e Tecnologie BiomedicheUniversità di CagliariMonserratoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-593-4
  • Online ISBN 978-1-60761-594-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site