RNA Interference

From Biology to Clinical Applications

  • Wei-Ping Min
  • Thomas Ichim

Part of the Methods in Molecular Biology book series (MIMB, volume 623)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Physiology of RNA Interference

    1. Front Matter
      Pages 1-1
    2. Michael P. Gantier, Bryan R. G. Williams
      Pages 21-33
    3. Dominique L. Ouellet, Patrick Provost
      Pages 35-65
    4. Hirohiko Hohjoh
      Pages 67-79
    5. Pirkko Muhonen, Harry Holthofer
      Pages 93-107
    6. Yasuhito Ishigaki, Akihiro Nagao, Tsukasa Matsunaga
      Pages 109-121
    7. Ivo L. Hofacker, Hakim Tafer
      Pages 137-154
    8. Joachim W. Engels, Dalibor Odadzic, Romualdas Smicius, Jens Haas
      Pages 155-170
  3. RNA Interference in the Laboratory and siRNA Delivery

    1. Front Matter
      Pages 172-172
    2. Xiufen Zheng, Costin Vladau, Aminah Shunner, Wei-Ping Min
      Pages 173-188
    3. Piotr G. Rychahou, B. Mark Evers
      Pages 189-195
    4. Satoshi Fujita, Kota Takano, Eiji Ota, Takuma Sano, Tomohiro Yoshikawa, Masato Miyake et al.
      Pages 197-209
    5. Satya Prakash, Meenakshi Malhotra, Venkatesh Rengaswamy
      Pages 211-229
    6. Jin-Mei Lai, Chi-Ying F. Huang, Chang-Han Chen
      Pages 231-242
    7. Dhananjay J. Jere, Chong-Su Cho
      Pages 243-269
    8. Jose L. R. Brito, Nicola Brown, Gareth J. Morgan
      Pages 299-309
  4. Clinical Implementation

    1. Front Matter
      Pages 312-312
    2. Chih-Ping Mao, T.-C. Wu
      Pages 325-339
    3. Zhu-Xu Zhang, Marianne E. Beduhn, Xiufen Zheng, Wei-Ping Min, Anthony M. Jevnikar
      Pages 341-355
    4. Xusheng Zhang, Mu Li, Wei-Ping Min
      Pages 357-371
    5. Miyuki Azuma, Patcharee Ritprajak, Masaaki Hashiguchi
      Pages 373-381
    6. Philippe Sarret, Louis Doré-Savard, Nicolas Beaudet
      Pages 383-395
    7. Shaguna Seth, Michael V. Templin, Gregory Severson, Oleksandr Baturevych
      Pages 397-422
  5. Back Matter
    Pages 437-450

About this book


From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace. In RNA Interference: From Biology to Clinical Applications, expert contributors provide an overview of the most current science and protocols that span the biological disciplines from detailed nucleic acid chemistry, to pharmacology, to the manipulation of signal transduction pathways. Divided into three distinct sections, this volume delves into the physiology of RNA interference, RNA interference in the laboratory and siRNA delivery, and preclinical and clinical issues associated with the use of RNAi-inducing agents as drugs in order to stimulate new questions and offer the tools necessary to start addressing those questions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and inspiring, RNA Interference: From Biology to Clinical Applications aims to promote and motivate innovation by reviewing what has been done, providing details of how it has been done, and encouraging speculation on what the future may hold.


Gene silencing Laboratory RNAi-inducing agents Therapeutics and drugs Viral defense physiology siRNA

Editors and affiliations

  • Wei-Ping Min
    • 1
  • Thomas Ichim
    • 2
  1. 1.Dept. Surgery, Pathology & OncologyUniversity of Western OntarioLondonCanada
  2. 2.MediStem Laboratories, Inc.TempeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-587-3
  • Online ISBN 978-1-60761-588-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site