Cytoskeleton Methods and Protocols

  • Ray H. Gavin

Part of the Methods in Molecular Biology book series (MIMB, volume 586)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Live-Cell Imaging of the Cytoskeleton

    1. Front Matter
      Pages 1-1
    2. Sarah Woolner, Ann L. Miller, William M. Bement
      Pages 23-39
  3. Dynamics of Actin Filaments and Microtubules

    1. Front Matter
      Pages 70-70
  4. Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components

    1. Front Matter
      Pages 116-116
    2. Alessandro Esposito, Simon Schlachter, Gabriele S. Kaminski Schierle, Alan D. Elder, Alberto Diaspro, Fred S. Wouters et al.
      Pages 117-142
    3. Robert F. Hennigan, Mary F. Chaiken, Lauren A. Foster, Wallace Ip
      Pages 143-156
    4. Julia Dyachok, Cheol-Min Yoo, Karuppaiah Palanichelvam, Elison B. Blancaflor
      Pages 157-169
    5. Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon
      Pages 171-184
  5. Electron Microscopy Protocols for Imaging the Cytoskeleton

    1. Front Matter
      Pages 186-186
    2. Andreas Holzinger, Eiko Kawamura, Geoffrey O. Wasteneys
      Pages 243-262
  6. Analysis of Cell and Organelle Motility

    1. Front Matter
      Pages 264-264
    2. Vidyalakshmi Rajagopalan, Elizabeth O. Corpuz, Mark J. Hubenschmidt, Caroline R. Townsend, David J. Asai, David E. Wilkes
      Pages 283-299
    3. Deborah Wessels, Spencer Kuhl, David R. Soll
      Pages 315-335
    4. Charles B. Lindemann, Kathleen A. Lesich
      Pages 337-353
  7. Genetic and Proteomics Approaches for Cytoskeleton Protein Function

  8. Back Matter
    Pages 389-390

About this book


In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology™ series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls.

Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.


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Editors and affiliations

  • Ray H. Gavin
    • 1
  1. 1.Brooklyn CollegeCity University of New YorkNew YorkU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-375-6
  • Online ISBN 978-1-60761-376-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site