Heterologous Expression of Membrane Proteins

Methods and Protocols

  • Isabelle Mus-Veteau

Part of the Methods in Molecular Biology™ book series (MIMB, volume 601)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Annie Frelet-Barrand, Sylvain Boutigny, Edmund R. S. Kunji, Norbert Rolland
    Pages 67-85
  3. Olivier Joubert, Rony Nehmé, Michel Bidet, Isabelle Mus-Veteau
    Pages 87-103
  4. Valérie Panneels, Irmgard Sinning
    Pages 135-147
  5. Birgit Schneider, Friederike Junge, Vladimir A. Shirokov, Florian Durst, Daniel Schwarz, Volker Dötsch et al.
    Pages 165-186
  6. Katia Duquesne, James N. Sturgis
    Pages 205-217
  7. Back Matter
    Pages 269-272

About this book

Introduction

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field. 

Keywords

Crystallization Escherichia coli Heterologous expression systems Protein solubilization Stabilization membrane proteins molecular biology proteins saccharomyces cerevisiae virus

Editors and affiliations

  • Isabelle Mus-Veteau
    • 1
  1. 1.CNRS - UMR 6543Université NiceNice CX 02France

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-344-2
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-343-5
  • Online ISBN 978-1-60761-344-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book