Ligand-Macromolecular Interactions in Drug Discovery

Methods and Protocols

  • Ana Cecília A. Roque

Part of the Methods in Molecular Biology book series (MIMB, volume 572)

Table of contents

  1. Front Matter
    Pages 1-9
  2. Reviews

    1. Front Matter
      Pages 1-1
    2. Ana Sofia Pina, Abid Hussain, Ana Cecília A. Roque
      Pages 3-12
    3. Tammy Nolan, Nidhi Singh, Christopher R. McCurdy
      Pages 13-29
    4. Ana Luísa Carvalho, José Trincão, Maria João Romão
      Pages 31-56
    5. Nuno M. F. S. A. Cerqueira, Sérgio F. Sousa, Pedro A. Fernandes, Maria João Ramos
      Pages 57-70
  3. Protocols

    1. Front Matter
      Pages 72-72
    2. Rolf Breinbauer, Matthias Mentel
      Pages 73-80
    3. Aldino Viegas, Anjos L. Macedo, Eurico J. Cabrita
      Pages 81-100
    4. Gisbert Schneider, Andreas Schüller
      Pages 135-147
    5. Shuguang Liang, Wei Xu, Kurumi Y. Horiuchi, Yuan Wang, Haiching Ma
      Pages 149-160
    6. Lihao Meng, Dawn Mattoon, Paul Predki
      Pages 177-188
    7. Milena Quaglia, Ersilia De Lorenzi
      Pages 189-202
    8. Maria Minunni, Anna Rita Bilia
      Pages 203-218
  4. Back Matter
    Pages 1-7

About this book


Drug research has been greatly transformed by the "omics revolution" and advances in computational tools, combinatorial chemistry, and high throughput screening techniques (HTS). These implements have advanced the ability to identify target molecules that serve as points of attack for future medicines, the design and synthesis of potential lead compounds, and further characterization, screening, and assays for therapeutic efficacy and toxicity. In Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols, experts in the field highlight the main principles and methodologies currently utilized in the study of molecular interactions between compounds, either natural or synthetic, and complementary biological targets, within the scope of drug discovery. Beginning with a historical perspective of drug research focusing on the contribution of genomics, proteomics, high-throughput methods, and computational developments in drug discovery, the book then delves into highly detailed methods on topics such as NMR spectroscopy, compound library design and synthesis, chemical and biological microarrays, and many others.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols will serve as an ideal guide to scientists in academia and in industry who are striving to further our knowledge of medicines.


Biological microarrays Compound libraries Drug research and design High-throughput methods Proteins Spin Target molecules Titration biology chemistry lead

Editors and affiliations

  • Ana Cecília A. Roque
    • 1
  1. 1.Fac. Ciências e Tecnologia, Depto. QuímicaUniversidade Nova de LisboaCaparicaPortugal

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-243-8
  • Online ISBN 978-1-60761-244-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site