Molecular Epidemiology of Microorganisms

Methods and Protocols

  • Dominique A.  Caugant
Part of the Methods in Molecular Biology™ book series (MIMB, volume 551)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michel Tibayrenc
    Pages 1-12
  3. Timothy J. Johnson, Lisa K. Nolan
    Pages 27-35
  4. Alex van Belkum, Elisabeth van Pelt-Verkuil, John P. Hays
    Pages 37-47
  5. David J. Ecker, Christian Massire, Lawrence B. Blyn, Steven A. Hofstadler, James C. Hannis, Mark W. Eshoo et al.
    Pages 71-87
  6. Norman K. Fry, Paul H.M. Savelkoul, Paolo Visca
    Pages 89-104
  7. Ana Belén Ibarz Pavón, Martin C.J. Maiden
    Pages 129-140
  8. Gilles Vergnaud, Christine Pourcel
    Pages 141-158
  9. Ed J. Kuijper, Renate J. van den Berg, Jon S. Brazier
    Pages 159-171
  10. Pablo Bifani, Natalia Kurepina, Barun Mathema, Xiao-Ming Wang, Barry Kreiswirth
    Pages 173-188
  11. Marie Hallin, Alexander W. Friedrich, Marc J. Struelens
    Pages 189-202
  12. Carol Holm-Hansen, Kirsti Vainio
    Pages 203-215
  13. Hervé Tettelin, Tamara Feldblyum
    Pages 231-247
  14. Stephan Huehn, Burkhard Malorny
    Pages 249-285

About this book

Introduction

The development of molecular genotyping methods has revolutionized the possibility for classification of microorganisms at the sub-species level. This investigation of species diversity is crucial for deciding the molecular relatedness of isolates for epidemiological studies. In Molecular Epidemiology of Microorganisms: Methods and Protocols, readers will find readily reproducible methods for determining the molecular epidemiology of microorganisms, all of which provide effective discrimination of unrelated strains. This volume covers a wide range of techniques which can be easily applied to the investigation of transmissible diseases, directing researchers towards the most effective methods based on the particular information to be obtained. Describing both traditional and novel techniques, expert researchers present a series of methods-based chapters with applications to some of the most important microbes. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Molecular Epidemiology of Microorganisms: Methods and Protocols provides state-of-the-art techniques which will prove to be critical in unraveling the routes of spread of pathogens for humans, animals, and plants.

Keywords

Bacterial genome sequencing Epidemiological Epidemiology Multilocus sequence typing Multiple locus VNTR Pulsed-field gel electrophoresis Randomly amplified DNA analysis Rep-PCR Spa-typing Viral gene sequencing molecular epidemiology

Editors and affiliations

  • Dominique A.  Caugant
  1. 1.Norwegian Institute of Public HealthOsloNorway

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-999-4
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-998-7
  • Online ISBN 978-1-60327-999-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book