Single Nucleotide Polymorphisms

Methods and Protocols

  • Anton A. Komar

Part of the Methods in Molecular Biology™ book series (MIMB, volume 578)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction to Single Nucleotide Polymorphisms

    1. Front Matter
      Pages 1-1
    2. Barkur S. Shastry
      Pages 3-22
    3. Ryan Hunt, Zuben E. Sauna, Suresh V. Ambudkar, Michael M. Gottesman, Chava Kimchi-Sarfaty
      Pages 23-39
  3. Bioinformatic Analysis of SNPs

    1. Front Matter
      Pages 41-41
    2. Christopher Phillips
      Pages 43-71
  4. SNP Identification and Detection Strategies: Whole Genome Sequencing and Re-Sequencing

    1. Front Matter
      Pages 93-93
    2. Jose Luis Royo, Jose Jorge Galán
      Pages 123-133
  5. Prescreening (Melting Based) Methods for SNP Discovery and Analysis

  6. Prescreening (Conformation Based) Methods for SNP Discovery and Analysis

    1. Front Matter
      Pages 167-167
    2. Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike
      Pages 169-182
    3. Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike
      Pages 183-192
    4. Tomoko Tahira, Yoji Kukita, Koichiro Higasa, Yuko Okazaki, Aki Yoshinaga, Kenshi Hayashi
      Pages 193-207
    5. Igor A. Prokhorenko, Irina V. Astakhova, Kuvat T. Momynaliev, Timofei S. Zatsepin, Vladimir A. Korshun
      Pages 209-222
    6. Tania Tabone, Georgina Sallmann, Richard G. H. Cotton
      Pages 223-234

About this book


In recent years, single nucleotide polymorphisms have received increased and special attention in a rapidly developing field of personalized medicine and drug treatment. Comprising more than eighty percent of all known polymorphisms, single nucleotide polymorphisms (SNPs) are primarily responsible for phenotypic differences between individuals, and have been suggested to affect the development of diseases in humans and the response to drug treatment and environmental stress. In Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition, expert researchers explore the latest advances in this area, highlighting the substantial progress that has been made in SNP genotyping, examining recent developments in high-throughput genotyping approaches, and exploring our new understanding of the impact of SNPs on gene function. Chapters address the impact of SNPs on phenotype, examine SNP databases, look at methods that have been applied for SNP bioinformatics discovery and analysis, and discuss advanced experimental approaches used for SNP detection. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Current and innovative, Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition is an essential guidebook for individual researchers as well as institutions and companies working in the field.


Advanced genome sequencing Allele DNA DNA sequencing Direct SNP detection Indirect SNP detection Isothermal smart amplification PCR SNP SNP databases Single Nucleotide Polymorphism bioinformatics genes hybridization molecular biology

Editors and affiliations

  • Anton A. Komar
    • 1
  1. 1.Center for Gene Regulation inCleveland State UniversityClevelandU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-410-4
  • Online ISBN 978-1-60327-411-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book