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Enzymatic Analysis

A Practical Guide

  • Janet V. Passonneau
  • Oliver H. Lowry
Book

Table of contents

  1. Front Matter
    Pages i-x
  2. General Principles

    1. Front Matter
      Pages 2-2
    2. Janet V. Passonneau, Oliver H. Lowry
      Pages 3-21
    3. Janet V. Passonneau, Oliver H. Lowry
      Pages 23-49
    4. Janet V. Passonneau, Oliver H. Lowry
      Pages 51-69
    5. Janet V. Passonneau, Oliver H. Lowry
      Pages 71-81
  3. Specific Methods and Procedures

    1. Front Matter
      Pages 84-84
    2. Janet V. Passonneau, Oliver H. Lowry
      Pages 85-110
    3. Janet V. Passonneau, Oliver H. Lowry
      Pages 111-228
    4. Janet V. Passonneau, Oliver H. Lowry
      Pages 229-305
  4. Quantitative Histochemistry

    1. Front Matter
      Pages 320-320
    2. Janet V. Passonneau, Oliver H. Lowry
      Pages 321-328
    3. Janet V. Passonneau, Oliver H. Lowry
      Pages 329-336
    4. Janet V. Passonneau, Oliver H. Lowry
      Pages 337-351
    5. Janet V. Passonneau, Oliver H. Lowry
      Pages 353-362
  5. Back Matter
    Pages 365-403

About this book

Introduction

Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.

Keywords

enzyme enzymes NADP Nucleotide

Authors and affiliations

  • Janet V. Passonneau
    • 1
  • Oliver H. Lowry
    • 2
  1. 1.WashingtonUSA
  2. 2.Washington UniversitySt. LouisUSA

Bibliographic information