Peptide Microarrays

Methods and Protocols

  • Marina Cretich
  • Marcella Chiari

Part of the Methods in Molecular Biology™ book series (MIMB, volume 570)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Victor E. Tapia, Bernhard Ay, Rudolf Volkmer
      Pages 3-17
    3. Alexandra Thiele, Johannes Zerweck, Mike Schutkowski
      Pages 19-65
  3. Applications

    1. Front Matter
      Pages 155-155
    2. Dirk F.H. Winkler, Kai Hilpert, Ole Brandt, Robert E.W. Hancock
      Pages 157-174
    3. Douglas J. Briant, James M. Murphy, Genie C. Leung, Frank Sicheri
      Pages 175-185
    4. Genie C. Leung, James M. Murphy, Doug Briant, Frank Sicheri
      Pages 187-195
    5. Alexandra Thiele, Johannes Zerweck, Matthias Weiwad, Gunter Fischer, Mike Schutkowski
      Pages 203-219
    6. Marina Cretich, Renato Longhi, Angelo Corti, Francesco Damin, Gabriele Di Carlo, Valentina Sedini et al.
      Pages 221-232
    7. Chris R. Taitt, Stella H. North, Nadezhda V. Kulagina
      Pages 233-255
    8. Alan Rigter, Jan Priem, Drophatie Timmers-Parohi, Jan Pm Langeveld, Alex Bossers
      Pages 257-271
  4. Technological Advancements

    1. Front Matter
      Pages 285-285
    2. Vianney Souplet, Clément Roux, Oleg Melnyk
      Pages 287-297
    3. Francois Debaene, Nicolas Winssinger
      Pages 299-307
    4. Mario Beyer, Ines Block, Kai König, Alexander Nesterov, Simon Fernandez, Thomas Felgenhauer et al.
      Pages 309-316

About this book

Introduction

Due to their versatility, along with the diminishing costs of library synthesis and the growth of commercial support, peptide microarrays will likely expand beyond being just a research tool into an adaptable and powerful platform to be harnessed for wider drug discovery and point-of-care applications. In Peptide Microarrays: Methods and Protocols, experts in the field provide a cutting-edge view of peptide array technology, its applications, and technical issues. After examining how peptides can characterize proteins and clarify, at the amino acid level, the molecular recognition events in which they are involved, the volume goes on to cover topics such as the production and use of peptide arrays for enzyme and binding motifs characterization, epitope mapping, and diagnostics, the newest technological advancements, as well as software and web tools for the design of peptide arrays and for the analysis of output data. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Peptide Microarrays: Methods and Protocols promises to serve scientists with its unique insights and novel solutions in peptide array technology in order to advance this vital field.

Keywords

Binding motif characterization Drug discovery Epitope mapping Library synthesis Microarray Parallel analysis Protein interactions Small molecule ligands Termination Web tools molecular biology

Editors and affiliations

  • Marina Cretich
    • 1
  • Marcella Chiari
    • 2
  1. 1.Inst. Chimica del RiconoscimentoCNRMilanoItaly
  2. 2.Inst. Chimica del RiconoscimentoCNRMilanoItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-394-7
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-393-0
  • Online ISBN 978-1-60327-394-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book