Molecular Biomethods Handbook

  • John M. Walker
  • Ralph Rapley

Table of contents

  1. Front Matter
    Pages i-xx
  2. Nucleic Acid Methods

    1. Gayle Corkill, Ralph Rapley
      Pages 3-15
    2. Gareth J. S. Jenkins
      Pages 17-27
    3. Marilena Aquino de Muro
      Pages 41-53
    4. Bronwen M. Harvey, Pirkko Soundy
      Pages 55-64
    5. Beatriz Sanchez-Vega
      Pages 65-87
    6. Jeroen H. Roelfsema, Dorien J. M. Peters
      Pages 107-115
    7. Kim Hung Leung, Shea Ping Yip
      Pages 117-131
    8. Lúcia Maria da Cunha Galvão, Eliane Lages-Silva
      Pages 133-147
    9. David Sugden, Patricia de Winter
      Pages 149-168
    10. Anu Suomalainen, Ann-Christine Syvänen
      Pages 169-178
    11. Carolina Sismani, Ludmila Kousoulidou, Philippos C. Patsalis
      Pages 179-193
    12. Arnis Druka, Robbie Waugh, Pete Hedley
      Pages 195-211
    13. Peng-Hui Wang, Yann-Jang Chen, Chi-Hung Lin
      Pages 213-226
    14. Craig Winstanley
      Pages 227-238
    15. Jane Bayani, Jeremy A. Squire
      Pages 239-255
    16. Phillip G. Febbo
      Pages 269-289

About this book


Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up-to-date collection of methods used regularly in the authors’ own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.


DNA Nucleotide PCR bioinformatics biomethods cryopreservation electron microscopy gene expression genes hybridization molecular biology nucleic acid biomethods protein and cell biomethods proteins tissue

Editors and affiliations

  • John M. Walker
    • 1
  • Ralph Rapley
    • 1
  1. 1.School of Life SciencesUniversity of HertfordshireHatfield, HertfordshireUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-370-1
  • Online ISBN 978-1-60327-375-6
  • About this book