PCR Detection of Microbial Pathogens

  • Mark Wilks

Part of the Methods in Molecular Biology book series (MIMB, volume 943)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Gemma Johnson, Tania Nolan, Stephen A. Bustin
    Pages 1-16
  3. Johannes Hedman, Peter Rådström
    Pages 17-48
  4. Paul S. Wallace, William G. MacKay
    Pages 49-79
  5. Guma M. K. Abdeldaim, Björn Herrmann
    Pages 115-123
  6. Jonas M. Winchell, Stephanie L. Mitchell
    Pages 149-158
  7. Renate Johanna van den Berg, Dennis Bakker, Ed J. Kuijper
    Pages 247-256
  8. Emiko Rimbara, Masanori Sasatsu, David Y. Graham
    Pages 279-287

About this book

Introduction

PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one’s own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first  chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered  in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.

Keywords

PCR detection bacterial pathogens clinical specimens molecular microbiology real-time PCR

Editors and affiliations

  • Mark Wilks
    • 1
  1. 1.Deptartment of MicrobiologySt. Bartholomew's HospitalLondonUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-353-4
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-352-7
  • Online ISBN 978-1-60327-353-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book