Cell-Free Protein Production

Methods and Protocols

  • Yaeta Endo
  • Kazuyuki Takai
  • Takuya Ueda

Part of the Methods in Molecular Biology book series (MIMB, volume 607)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Yoshihiro Shimizu, Takuya Ueda
    Pages 11-21
  3. Kazuyuki Takai, Yaeta Endo
    Pages 23-30
  4. Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, Eiji Ando
    Pages 31-42
  5. Satoshi Mikami, Tominari Kobayashi, Hiroaki Imataka
    Pages 43-52
  6. Takafumi Tsuboi, Satoru Takeo, Tatsuya Sawasaki, Motomi Torii, Yaeta Endo
    Pages 73-83
  7. Takuya Kanno, Yuzuru Tozawa
    Pages 85-99
  8. Shin-ichi Makino, Michael A. Goren, Brian G. Fox, John L. Markley
    Pages 127-147
  9. Miki Watanabe, Ken-ichi Miyazono, Masaru Tanokura, Tatsuya Sawasaki, Yaeta Endo, Ichizo Kobayashi
    Pages 149-160
  10. Sina Reckel, Solmaz Sobhanifar, Florian Durst, Frank Löhr, Vladimir A. Shirokov, Volker Dötsch et al.
    Pages 187-212
  11. Akira Nozawa, Hideaki Nanamiya, Yuzuru Tozawa
    Pages 213-218
  12. Takuya Ueda, Takashi Kanamori, Hiroyuki Ohashi
    Pages 219-225
  13. Takeshi Sunami, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo
    Pages 243-256
  14. Back Matter
    Pages 257-261

About this book


During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.


DNA Laboratory Microarray Termination Translation membrane proteins molecular biology tRNA

Editors and affiliations

  • Yaeta Endo
    • 1
  • Kazuyuki Takai
    • 2
  • Takuya Ueda
    • 3
  1. 1.Ehime UniversityMatsuyamaJapan
  2. 2.Cell-Free Sciene & TechnologyEhime UniversityEhimeJapan
  3. 3.Graduate School of Frontier SciencesUniversity of TokyoKashiwa, ChibaJapan

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-330-5
  • Online ISBN 978-1-60327-331-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site