Gene Therapy Protocols

Design and Characterization of Gene Transfer Vectors

  • Joseph M. Le Doux

Part of the Methods in Molecular Biology™ book series (MIMB, volume 434)

Table of contents

  1. Front Matter
    Pages i-xii
  2. María de las Mercedes Segura, Amine Kamen, Alain Garnier
    Pages 1-11
  3. John O. Konz, Lee R. Pitts, Sangeetha L. Sagar
    Pages 13-23
  4. Fubao Wang, Bill C. Mathis, Allison Montalvo, Jayanthi J. Wolf, John A. Lewis
    Pages 25-36
  5. Richard H. Smith, Linda Yang, Robert M. Kotin
    Pages 37-54
  6. Salvador F. Ausar, Sangeeta B. Joshi, C. Russell Middaugh
    Pages 55-80
  7. Hongju Wu, David T. Curiel
    Pages 113-132
  8. Catherine R., O’ Riordan, Antonius Song
    Pages 133-160
  9. Anette Bonsted, Ernst Wagner, Lina Prasmickaite, Anders HØgset, Kristian Berg
    Pages 171-181
  10. Ali Ramezani, Teresa S. Hawley, Robert G. Hawley
    Pages 183-203
  11. Changyu Zheng, Bruce J. Baum
    Pages 205-219
  12. James F. Curtin, Marianela Candolfi, Mariana Puntel, Weidong Xiong, A.K.M. Muhammad, Kurt Kroeger et al.
    Pages 239-266
  13. Lalitha R. Belur, R. Scott McIvor, Andrew Wilber
    Pages 267-276
  14. Toni Cathomen, David J. Segal, Vincent Brondani, Felix Müller-Lerch
    Pages 277-290
  15. Rajagopal Ramesh
    Pages 301-331
  16. Pedro Lei, Stelios T. Andreadis
    Pages 367-380
  17. Back Matter
    Pages 311-313

About this book


In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.


DNA Promoter Virus gene expression gene therapy gene transfer gene transfer vectors genes tissue transcription transgene expression

Editors and affiliations

  • Joseph M. Le Doux
    • 1
  1. 1.Department of Biomedical EngineeringGeorgia Institute of Technology and Emory UniversityGA 30322-0355

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-247-6
  • Online ISBN 978-1-60327-248-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site