Protein Folding, Misfolding, and Disease

Methods and Protocols

  • Andrew F. Hill
  • Kevin J. Barnham
  • Stephen P. Bottomley
  • Roberto Cappai

Part of the Methods in Molecular Biology book series (MIMB, volume 752)

Table of contents

  1. Front Matter
    Pages i-x
  2. Luke A. Miles, Gabriela A. N. Crespi, Sen Han, Andrew F. Hill, Michael W. Parker
    Pages 17-28
  3. Denis B. Scanlon, John A. Karas
    Pages 29-43
  4. Jennifer Phan, Nasrin Yamout, Jason Schmidberger, Stephen P. Bottomley, Ashley M. Buckle
    Pages 45-57
  5. Lisa D. Cabrita, Christopher A. Waudby, Christopher M. Dobson, John Christodoulou
    Pages 97-120
  6. Danny M. Hatters, Michael D. W. Griffin
    Pages 121-136
  7. Tuomas P. J. Knowles, Glyn L. Devlin, Christopher M. Dobson, Mark E. Welland
    Pages 137-145
  8. Sally L. Gras, Adam M. Squires
    Pages 147-163
  9. John D. Gehman, Frances Separovic
    Pages 165-177
  10. Chi Le Lan Pham, Yee-Foong Mok, Geoffrey J. Howlett
    Pages 179-196
  11. Sally L. Gras, Lynne J. Waddington, Kenneth N. Goldie
    Pages 197-214
  12. Back Matter
    Pages 241-249

About this book


Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease.  In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of current methods for studying the analysis of protein folding and misfolding, featuring strategies for expressing and refolding recombinant proteins which can then be utilized in subsequent experiments. This detailed volume also covers methods for analyzing the formation of amyloid, protocols for determining the size and structure of native and misfolded proteins, as well as specific examples of where misfolded proteins can be examined using state-of –the-art technologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Up to date and authoritative, Protein Folding, Misfolding, and Disease: Methods and Protocols offers researchers the tools necessary to move ahead in this vital field.


Amyloid formation Human disorders Misfolded proteins Native proteins Protein structures Recombinant proteins

Editors and affiliations

  • Andrew F. Hill
    • 1
  • Kevin J. Barnham
    • 2
  • Stephen P. Bottomley
    • 3
  • Roberto Cappai
    • 4
  1. 1.Bio21 Molecular Science and, Biotechnology InstituteUniversity of MelbourneParkvilleAustralia
  2. 2.Bio21 Molecular Science and, Biotechnology InstituteUniversity of MelbourneParkvilleAustralia
  3. 3., Department of Biochemistry &Monash UniversityClaytonAustralia
  4. 4.Bio21 Molecular Science and, Biotechnology InstituteUniversity of MelbourneParkvilleAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-221-6
  • Online ISBN 978-1-60327-223-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site