Microarray Analysis of the Physical Genome

Methods and Protocols

  • Jonathan R. Pollack

Part of the Methods in Molecular Biology™ book series (MIMB, volume 556)

Table of contents

  1. Front Matter
    Pages i-x
  2. Jonathan R. Pollack
    Pages 1-6
  3. Bradley P. Coe, William W. Lockwood, Raj Chari, Wan L. Lam
    Pages 7-19
  4. Young H. Kim, Jonathan R. Pollack
    Pages 21-32
  5. Hanlee Ji, Katrina Welch
    Pages 67-87
  6. Daniel E. Deatherage, Dustin Potter, Pearlly S. Yan, Tim H.-M. Huang, Shili Lin
    Pages 117-139
  7. Emily A. Vucic, Ian M. Wilson, Jennifer M. Campbell, Wan L. Lam
    Pages 141-153
  8. X. Shirley Liu, Clifford A. Meyer
    Pages 165-175
  9. Yoichiro Shibata, Gregory E. Crawford
    Pages 177-190
  10. Neerja Karnani, Christopher M. Taylor, Anindya Dutta
    Pages 191-203
  11. Keyan Salari, Jonathan R. Pollack
    Pages 205-216
  12. Back Matter
    Pages 217-223

About this book


In the early years of microarray technology, efforts were directed mainly at profiling expressed genes, while recently the microarray platform has been adapted into diverse applications directed toward the investigation of the physical genome. In Microarray Analysis of the Physical Genome: Methods and Protocols, experts in the field cover DNA microarray applications for the detection and characterization of genomic DNA-associated copy number alteration, loss of heterozygosity (LOH), cytosine methylation, protein binding sites, regulatory elements, and replication timing, with an emphasis on higher eukaryote (animal) and cancer genomes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include a brief introduction to their respective subjects, lists of the necessary materials and reagents, step-by-step protocols, and time-saving notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Microarray Analysis of the Physical Genome: Methods and Protocols is of great value to the molecular biologist or computational biologist interested in understanding the principles of these analyses or in planning future experiments using microarrays to characterize the physical genome.


BAC Cancer genomes Cytosine methylation DNA Heterozygosity (LOH) Higher eukaryote genomes Microarray Protein binding sites Replication timing eukaryote genes hybridization molecular biology

Editors and affiliations

  • Jonathan R. Pollack

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-191-2
  • Online ISBN 978-1-60327-192-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site