Book Volume 420 2008

Drosophila

Methods and Protocols

Editors:

ISBN: 978-1-58829-817-1 (Print) 978-1-59745-583-1 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-xi

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    Pages 1-25

    Drosophila melanogaster and the Development of Biology in the 20th Century

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    Pages 27-44

    Getting Started

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    Pages 45-59

    FlyBase

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    Pages 61-77

    The Use of P-Element Transposons to Generate Transgenic Flies

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    Protocol

    Pages 79-95

    The GAL4 System

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    Pages 97-117

    P-Element Mutagenesis

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    Pages 119-138

    EMS Screens

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    Pages 139-153

    RNAi Screening in Cultured Drosophila Cells

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    Pages 155-174

    Methods for Homologous Recombination in Drosophila

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    Pages 175-195

    Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis

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    Pages 197-205

    Cuticle Preparation of Drosophila Embryos and Larvae

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    Pages 207-218

    Immunolabeling of Embryos

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    Pages 219-238

    Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins

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    Pages 239-251

    Analysis of Neuromuscular Junctions

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    Pages 253-263

    Immunolabeling of Imaginal Discs

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    Pages 265-275

    Imaging Drosophila Pupal Wing Morphogenesis

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    Pages 277-287

    Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections

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    Pages 289-302

    Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues

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    Pages 303-317

    Linear RNA Amplification for the Production of Microarray Hybridization Probes

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    Protocol

    Pages 319-334

    An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs

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