Methods and Protocols

  • Andrew K. Ottens
  • Kevin K.W. Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 566)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Andrew K. Ottens
    Pages 1-21
  3. Disease Models in Neuroproteomics

    1. Front Matter
      Pages 23-23
    2. Jitendra R. Dave, Anthony J. Williams, Changping Yao, X.-C. May Lu, Frank C. Tortella
      Pages 25-40
    3. András Büki, Erzsébet Kövesdi, József Pál, Endre Czeiter
      Pages 41-55
    4. Anshu Chen, Joe E. Springer
      Pages 57-67
    5. Scott E. Hemby, Nilesh Tannu
      Pages 69-83
    6. Andrew T. N. Tebbenkamp, David R. Borchelt
      Pages 85-91
  4. Sub-Proteome Separations and Neuroproteomic Analysis

    1. Front Matter
      Pages 93-93
    2. Stefani N. Thomas, Diane Cripps, Austin J. Yang
      Pages 109-121
    3. Zhiqun Zhang, Firas H. Kobeissy, Andrew K. Ottens, Juan A. Martínez, Kevin K. W. Wang
      Pages 181-190
  5. Neuroproteomic Methodology and Bioinformatics

    1. Front Matter
      Pages 191-191
    2. Brian F. Fuller, Andrew K. Ottens
      Pages 193-200
    3. Tong Liu, Jun Hu, Hong Li
      Pages 201-216
    4. Firas H. Kobeissy, Zhiqun Zhang, Shankar Sadasivan, Mark S. Gold, Kevin K. W. Wang
      Pages 217-228
    5. Bingwen Lu, Tao Xu, Sung Kyu Park, Daniel B. McClatchy, Lujian Liao, John R. Yates III
      Pages 229-259
  6. Biofluid Analysis and Clinical Translation

    1. Front Matter
      Pages 261-261
    2. Hye Jin Hwang, Thomas Quinn, Jing Zhang
      Pages 263-276
    3. David M. Good, Joshua J. Coon
      Pages 277-291
    4. Monika W. Oli, Ronald L. Hayes, Gillian Robinson, Kevin K. W. Wang
      Pages 293-302
    5. Ronald L. Hayes, Gillian Robinson, Uwe Muller, Kevin K. W. Wang
      Pages 303-313
  7. Back Matter
    Pages 315-321

About this book


With the development of polypeptide ionization processes for mass spectrometry and the rapid processing of large-scale datasets with bioinformatics, researchers now have the tools necessary to propel neuroproteomics research to new levels. In Neuroproteomics: Methods and Protocols, experts in the neurological and analytical sciences present experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease. Divided into four convenient sections, this collection covers CNS animal models used for neuroproteomics research, methods for separating and analyzing discrete subcomponents of the neuroproteome, large-scale approaches for CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neuroproteomics: Methods and Protocols includes the clear "how-to" information needed to critically assess what can be accomplished in the field and how to best perform and evaluate neuroproteomic experiments in one’s own research.


Biofluid analysis Bioinformatics Biomarker research Central nervous system - CNS Clinical translation Disease models Mass spectrometry Nervous System Proteomics Sub-proteome separations Vivo glycoprotein proteins

Editors and affiliations

  • Andrew K. Ottens
    • 1
  • Kevin K.W. Wang
    • 2
  1. 1.Dept. Anatomy & NeurobiologyVirginia Commonwealth University School of MedicineRichmondU.S.A.
  2. 2.Center of Innovative ResearchBanyan Biomarkers, Inc. Center of Innovative ResearchAlachuaU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press, a part of Springer Science+Business Media, LLC 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-84-8
  • Online ISBN 978-1-59745-562-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site