Methods and Protocols

  • Alexander Serganov

Part of the Methods in Molecular Biology book series (MIMB, volume 540)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Benoit Heppell, Jérôme Mulhbacher, J. Carlos Penedo, Daniel A. Lafontaine
    Pages 25-37
  3. Alexander Mironov, Vitaly Epshtein, Evgeny Nudler
    Pages 39-51
  4. Jean-François Lemay, J. Carlos Penedo, Jérôme Mulhbacher, Daniel A. Lafontaine
    Pages 65-76
  5. Chad A. Brautigam, Catherine A. Wakeman, Wade C. Winkler
    Pages 77-95
  6. Olga Pikovskaya, Artem A. Serganov, Ann Polonskaia, Alexander Serganov, Dinshaw J. Patel
    Pages 115-128
  7. Daniel J. Klein, Adrian R. Ferré-D’Amaré
    Pages 129-139
  8. Jan Lipfert, Daniel Herschlag, Sebastian Doniach
    Pages 141-159
  9. Janina Buck, Boris Fürtig, Jonas Noeske, Jens Wöhnert, Harald Schwalbe
    Pages 161-171
  10. Tina M. Henkin
    Pages 207-214
  11. Clément Chevalier, Thomas Geissmann, Anne-Catherine Helfer, Pascale Romby
    Pages 215-232
  12. Claude Chiaruttini, Frédéric Allem, Mathias Springer
    Pages 233-245
  13. Pierre Fechter, Clément Chevalier, Gulnara Yusupova, Marat Yusupov, Pascale Romby, Stefano Marzi
    Pages 247-263
  14. Ilya Shamovsky, Evgeny Nudler
    Pages 265-279
  15. Günter Mayer, Michael Famulok
    Pages 291-300
  16. Sean A. Lynch, Shana Topp, Justin P. Gallivan
    Pages 321-333
  17. Laising Yen, Brent R. Stockwell, Richard C. Mulligan
    Pages 335-347
  18. Kristian H. Link, Ronald R. Breaker
    Pages 349-364
  19. Back Matter
    Pages 365-366

About this book


The revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues.  In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date coverage of various methods used to study riboswitches and other RNAs involved in gene expression control.  Examining biochemical and modern biophysical techniques, the volume focuses on mRNAs responding to small organic molecules but expands the definition of a riboswitch to incorporate classes of RNA that undergo conformational transitions in response to other stimuli in order to control the expression of genes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Riboswitches: Methods and Protocols promises to inspire both novices and experts working in a wide range of contemporary biological areas who wish to further develop their RNA methodology.


Ligation Termination Translation enzymes evolution gene expression genes molecular biology tRNA

Editors and affiliations

  • Alexander Serganov

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-88-6
  • Online ISBN 978-1-59745-558-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site